Ran binding protein 9 (RanBPM) binds IFN-λR1 in the IFN-λ signaling pathway

Autor: Jiajie Zhaoqiao, Junwen Zhang, Fusheng Liu, Bingren Huang, Hong Chen, Ruifang Mi, Xiaojie Cong, Guishan Jin, Meng Li, Xia Yang
Rok vydání: 2017
Předmět:
0301 basic medicine
Scaffold protein
Receptor complex
Immunoprecipitation
medicine.medical_treatment
Biology
Response Elements
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
Cell Line
Tumor
medicine
Humans
Receptors
Cytokine
Adaptor Proteins
Signal Transducing
Cell Line
Transformed
Receptors
Interferon
General Environmental Science
TNF Receptor-Associated Factor 6
Binding Sites
Interleukins
Intracellular Signaling Peptides and Proteins
Ubiquitination
Nuclear Proteins
Glutathione
Molecular biology
Interferon-Stimulated Gene Factor 3
gamma Subunit
Cell biology
Protein M
Cytoskeletal Proteins
HEK293 Cells
030104 developmental biology
Cytokine
chemistry
Interferons
Signal transduction
General Agricultural and Biological Sciences
Function (biology)
Protein Binding
Signal Transduction
Zdroj: Science China Life Sciences. 60:1030-1039
ISSN: 1869-1889
1674-7305
DOI: 10.1007/s11427-017-9028-6
Popis: Like the type I interferons (IFNs), the recently discovered cytokine IFN-λ displays antiviral, antiproliferative, and proapoptotic activities, mediated by a heterodimeric IFN-λ receptor complex composed of a unique IFN-λR1 chain and the IL-10R2 chain. However, the molecular mechanism of the IFN-λ-regulated pathway remains unclear. In this study, we newly identified RAN-binding protein M (RanBPM) as a binding partner of IFN-λR1. The interaction between RanBPM and IFN-λR1 was identified with a glutathione S-transferase pull-down assay and coimmunoprecipitation experiments. IFN-λ1 stimulates this interaction and affects the cellular distribution of RanBPM. However, the interaction between RanBPM and IFN-λR1 does not correlate with their conserved TRAF6-binding sites. Furthermore, we also found that RanBPM is a scaffolding protein with a modulatory function that regulates the activities of IFN-stimulated response elements. Therefore, RanBPM plays a novel role in the IFN-λ-regulated signaling pathway.
Databáze: OpenAIRE
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