The role of N-sulfation in the Nhydroxy-2-acetylaminofluorene-mediated outgrowth of diethylnitrosamine-initiated hepatocytes to γ-glutamyltranspeptidase-positive foci in male rat liver
| Autor: | E. Dinant Kroese, Gerard J. Mulder, Monique L.M. van de Poll, John H. N. Meerman |
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| Rok vydání: | 1988 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Sulfotransferase Pentachlorophenol Biology digestive system chemistry.chemical_compound Liver Neoplasms Experimental Sulfation Internal medicine polycyclic compounds medicine Animals Diethylnitrosamine Carcinogen Cocarcinogenesis Sulfates Bile duct Hydroxyacetylaminofluorene Rats Inbred Strains gamma-Glutamyltransferase General Medicine 2-Acetylaminofluorene Gamma glutamyltranspeptidase digestive system diseases Rats Endocrinology medicine.anatomical_structure Liver chemistry Hepatocyte Rat liver Sulfotransferases Precancerous Conditions Cell Division |
| Zdroj: | Carcinogenesis. 9:1953-1958 |
| ISSN: | 1460-2180 0143-3334 |
| DOI: | 10.1093/carcin/9.11.1953 |
| Popis: | The ability of N-hydroxy-2-acetylaminofluorene (N-OH-AAF) to promote the appearance of gamma-glutamyltranspeptidase-positive (GGT+) foci initiated by diethylnitrosamine (DEN) was studied in a slightly modified Solt and Farber protocol. This protocol consisted of the following treatments: initiation with a single dose of DEN followed by selection/promotion with several non-necrogenic doses of N-OH-AAF and partial hepatectomy. Treatment with N-OH-AAF resulted in a 25-fold increase of the liver volume occupied by GGT+ cells as compared to controls. The role of N-sulfation of N-OH-AAF in the GGT+ foci-selecting activity of N-OH-AAF was studied using the sulfotransferase inhibitor pentachlorophenol (PCP). Inhibition of the N-sulfation pathway with PCP during selection with N-OH-AAF resulted in a greater than 80% decrease in the volume occupied by GGT+ cells, without effects on the number of GGT+ foci generated with this protocol. Also, PCP reduced the number of so called oval and bile duct cells generated by the N-OH-AAF/partial hepatectomy treatment. It is concluded that N-sulfation of N-OH-AAF is responsible for the N-OH-AAF-mediated outgrowth of DEN-initiated hepatocytes to preneoplastic GGT+ foci. |
| Databáze: | OpenAIRE |
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