Lack of an effect of saxitonin on the contractility of isolated guinea-pig trachea, lung parenchyma and aorta
| Autor: | David R. Franz, Casey P. Robinson, Maria E. Bondura |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Carbachol Endothelium Guinea Pigs Biology In Vitro Techniques Toxicology Muscle Smooth Vascular Contractility chemistry.chemical_compound Norepinephrine Internal medicine medicine.artery medicine Animals heterocyclic compounds Lung Aorta Saxitoxin musculoskeletal neural and ocular physiology Muscle Smooth General Medicine Anatomy Acetylcholine Trachea medicine.anatomical_structure Endocrinology chemistry Endothelium Vascular medicine.symptom Histamine medicine.drug Muscle contraction Muscle Contraction |
| Zdroj: | Toxicology letters. 51(1) |
| ISSN: | 0378-4274 |
| Popis: | The effects of saxitonin were investigated in guinea-pig tracheal rings, lung parenchymal strips and aorta rings. Tracheal rings were used both with epithelium present and with it removed. Aorta rings were used both with endothelium present and with it removed. Saxitoxin, 1 pM to 0.1 microM, did not alter the resting tension of either airway tissues or aorta. Also 0.1 microM saxitonin did not reduce tension of tracheal rings contracted by 10 microM carbachol, parenchymal strips contracted by 100 microM acetylcholine or by 10 microM histamine, or aorta rings contracted by 10 microM norepinephrine. Responses of tracheal rings to 0.03-10 microM carbachol added cumulatively were not altered by 0.1 microM saxitoxin (EC50 with epithelium: 1.11 +/- 0.48 microM control, 2.01 +/- 0.71 microM with saxitoxin; EC50 without epithelium: 2.83 +/- 0.55 microM control, 2.05 +/- 0.58 microM with saxitoxin). Also, 1 microM saxitoxin did not alter contractions of parenchymal stirps to cumulatively added acetylcholine (EC50: 3.47 +/- 1.06 microM, control; 3.98 +/- 1.19 microM with saxitoxin), histamine (EC50: 0.83 +/- 0.24 microM, control; 0.60 +/- 0.13 microM with saxitoxin); or of aorta strips to norepinephrine with endothelium (EC50: 1.78 +/- 0.80 microM, control; 0.74 +/- 0.21 microM with saxitoxin) or without endothelium (EC50: 2.18 +/- 0.78 microM, control; 1.42 +/- 0.62 microM with saxitoxin). Thus, saxitoxin did not significantly alter contractile activity of airways of large arteries in vitro. |
| Databáze: | OpenAIRE |
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