Antioxidant, Anti-Inflammatory, and Antitumoral Effects of Aqueous Ethanolic Extract from Phoenix dactylifera L. Parthenocarpic Dates

Autor: Bassem Khemakhem, Naziha Marrakchi, Noureddine Drira, Zaineb Abdelkafi-Koubaa, M Chakroun, Hanen El Abed, Hafedh Mejdoub
Přispěvatelé: Laboratory of Plant Biotechnology [Sfax], Faculté des Sciences de Sfax, Université de Sfax - University of Sfax-Université de Sfax - University of Sfax, Laboratoire des Venins et Biomolécules Thérapeutiques - Laboratory of Venoms and Therapeutic Biomolecules (LR11IPT08), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université de Tunis El Manar (UTM), This research was supported by the Ministry of Higher Education and Scientific Research in Tunisia., The major part of this work was accomplished at the Faculty of Sciences of Sfax and at the Institute Pasteur of Tunis. The authors gratefully acknowledge the support of Mr. El Abed Mokhles for his assistance in the project.
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Antioxidant
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
DPPH
medicine.medical_treatment
[SDV]Life Sciences [q-bio]
Anti-Inflammatory Agents
lcsh:Medicine
Antioxidants
Mice
chemistry.chemical_compound
0302 clinical medicine
Tumor Cells
Cultured
MESH: Animals
Aqueous solution
Traditional medicine
biology
Chemistry
Phoeniceae
General Medicine
MESH: Phoeniceae
030220 oncology & carcinogenesis
MESH: Plant Extracts/pharmacology
Research Article
MESH: Ethanol
Article Subject
medicine.drug_class
General Biochemistry
Genetics and Molecular Biology
Anti-inflammatory
03 medical and health sciences
Phospholipase A2
In vivo
medicine
Animals
MESH: Tumor Cells
Cultured
IC50
MESH: Mice
MESH: Phospholipases A2/metabolism
Ethanol
General Immunology and Microbiology
Plant Extracts
lcsh:R
MESH: Antioxidants
MESH: Phospholipases A2/drug effects
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Phospholipases A2
030104 developmental biology
Phoenix dactylifera
biology.protein
MESH: Anti-Inflammatory Agents/pharmacology
Zdroj: BioMed Research International
BioMed Research International, Hindawi Publishing Corporation, 2018, 2018, pp.1542602. ⟨10.1155/2018/1542602⟩
BioMed Research International, Vol 2018 (2018)
ISSN: 2314-6133
2314-6141
DOI: 10.1155/2018/1542602⟩
Popis: The aim of this study was to evaluate the antioxidant, the anti-inflammatory, and the antitumoral activities of the aqueous ethanolic extract fromPhoenix dactyliferaL. parthenocarpic dates. The antioxidant activity was carried using DPPH radical scavenging activity. The result showed that parthenocarpic dates had strongly scavenging activity on DPPH reaching 94% with an IC50value of 0.15 ± 0.011 mg/mL (p < 0.05). The anti-inflammatory potential was determined by the inhibitory effect of the aqueous ethanolic extract on phospholipase A2activity as well as on carrageenan-induced paw oedema in mice. Thein vitrostudy showed that the extract inhibited the phospholipase A2activity with an IC50value of 130 μg/mL and thein vivostudy showed a significantly decrease in the paw oedema after 1 h compared to the control group. Finally, the antiproliferative activity of the aqueous ethanolic extract was assessed by MTT test against MCF-7 and MDA-MB-231 cancer cell lines. This extract was effective in inhibiting MDA-MB-231 and MCF-7 cancer cells growth with IC50values of 8 and 18 mg/mL, respectively, after 72 h treatment. These results confirm the ethnopharmacological significance ofPhoenix dactyliferaL. parthenocarpic dates, which could add support for its pharmaceutical use.
Databáze: OpenAIRE
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