Substrate analysis of homoserine acyltransferase from Bacillus cereus
| Autor: | Muzaffar Yusupov, Barney Bishop, Katharine Ziegler, Timothy L. Born |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Biophysics
Homoserine Bacillus cereus medicine.disease_cause Biochemistry Article Substrate Specificity Microbiology Iodoacetamide Nitrophenols chemistry.chemical_compound Methionine Diethyl Pyrocarbonate medicine Cloning Molecular Molecular Biology Escherichia coli chemistry.chemical_classification biology organic chemicals fungi Substrate (chemistry) Homoserine O-Succinyltransferase Cell Biology Hydrogen-Ion Concentration biology.organism_classification Enzyme Activation Kinetics Enzyme chemistry Cereus Acyltransferase bacteria Mutant Proteins |
| Zdroj: | Biochemical and Biophysical Research Communications. 361:510-515 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2007.07.044 |
| Popis: | Substrate specificity within the family of enzymes designated as homoserine transsuccinylases is variable, with some organisms utilizing succinyl-CoA and other organisms utilizing acetyl-CoA. In this study it is shown that the enzyme from Bacillus cereus uses acetyl-CoA as its acyl donor, but its catalytic rate is significantly lower than other HTS family members. BcHTS is inactivated by both iodoacetamide and diethyl pyrocarbonate and the enzyme can be partially protected from inactivation by the presence of succinyl-CoA. This leads to the conclusion that BcHTS can bind both acetyl-CoA and succinyl-CoA and suggests that it may represent an intermediate between the succinate-transferring HTS family members and the acetate-transferring HTS family members. The B. cereus enzyme was unable to rescue growth of an Escherichia coli strain lacking a functional transsuccinylase, however. |
| Databáze: | OpenAIRE |
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