Use of human perivascular stem cells for bone regeneration

Autor: Bruno Péault, Ronald K. Siu, Min Lee, Wei Yuan, Aaron W. James, Chia Soo, Asal Askarinam, Kang Ting, Victoria Scott, Virginia Nguyen, Raghav Goyal, Janette N. Zara, Mirko Corselli, Michael F. Chiang
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Pathology
Bone Regeneration
General Chemical Engineering
Mice
SCID
Mice
0302 clinical medicine
Tissue engineering
Osteogenesis
Femur
Medicine(all)
0303 health sciences
education.field_of_study
Tissue Scaffolds
Chemistry
General Neuroscience
Stem Cells
Stem Cell Biology
Anatomy
medicine.anatomical_structure
030220 oncology & carcinogenesis
Bone Defect
Models
Animal
CD146
Pericyte
Stem cell
Issue 63
medicine.medical_specialty
femoral defect
Neuroscience(all)
Population
Biomedical Engineering
Bioengineering
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Rats
Nude
Stem Cell
Immunology and Microbiology(all)
medicine
Animals
Humans
education
Bone regeneration
030304 developmental biology
General Immunology and Microbiology
Tissue Engineering
Biochemistry
Genetics and Molecular Biology(all)
Mesenchymal stem cell
Skull
calvarial defect
Rats
Chemical Engineering(all)
Pericytes
Parietal bone
Zdroj: James, A W, Zara, J N, Corselli, M, Chiang, M, Yuan, W, Nguyen, V, Askarinam, A, Goyal, R, Siu, R K, Scott, V, Lee, M, Ting, K, Péault, B & Soo, C 2012, ' Use of human perivascular stem cells for bone regeneration ', Journal of Visualized Experiments (JoVE), no. 63, pp. e2952 . https://doi.org/10.3791/2952
Journal of Visualized Experiments : JoVE
DOI: 10.3791/2952
Popis: Human perivascular stem cells (PSCs) can be isolated in sufficient numbers from multiple tissues for purposes of skeletal tissue engineering1-3. PSCs are a FACS-sorted population of 'pericytes' (CD146+CD34-CD45-) and 'adventitial cells' (CD146-CD34+CD45-), each of which we have previously reported to have properties of mesenchymal stem cells. PSCs, like MSCs, are able to undergo osteogenic differentiation, as well as secrete pro-osteogenic cytokines1,2. In the present protocol, we demonstrate the osteogenicity of PSCs in several animal models including a muscle pouch implantation in SCID (severe combined immunodeficient) mice, a SCID mouse calvarial defect and a femoral segmental defect (FSD) in athymic rats. The thigh muscle pouch model is used to assess ectopic bone formation. Calvarial defects are centered on the parietal bone and are standardly 4 mm in diameter (critically sized)8. FSDs are bicortical and are stabilized with a polyethylene bar and K-wires4. The FSD described is also a critical size defect, which does not significantly heal on its own4. In contrast, if stem cells or growth factors are added to the defect site, significant bone regeneration can be appreciated. The overall goal of PSC xenografting is to demonstrate the osteogenic capability of this cell type in both ectopic and orthotopic bone regeneration models.
Databáze: OpenAIRE
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