Photodynamic Anticancer Activities of Multifunctional Cobalt Ferrite Nanoparticles in Various Cancer Cells
| Autor: | Jin-Seung Jung, Han S. Uhm, Anser Ali, Kyong-Hoon Choi, Ho-Joong Kim, Bong Joo Park, Hyungbin Son, Eun Ha Choi, Joe Eun Min, Ki Chang Nam |
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| Rok vydání: | 2015 |
| Předmět: |
Materials science
Biocompatibility Stereochemistry medicine.medical_treatment Biomedical Engineering Metal Nanoparticles Pharmaceutical Science Medicine (miscellaneous) Antineoplastic Agents Apoptosis Bioengineering Photodynamic therapy Ferric Compounds HeLa Mice chemistry.chemical_compound Neoplasms Tumor Cells Cultured medicine Animals Humans General Materials Science Hematoporphyrin Photosensitizing Agents biology Cobalt biology.organism_classification Photochemotherapy chemistry Cancer cell MCF-7 Cells Cancer research Magnetic nanoparticles HeLa Cells Superparamagnetism |
| Zdroj: | Journal of Biomedical Nanotechnology. 11:226-235 |
| ISSN: | 1550-7033 |
| DOI: | 10.1166/jbn.2015.2031 |
| Popis: | To develop novel multifunctional magnetic nanoparticles (MNPs) with good magnetic properties, biocompatibility, and anticancer activities by photodynamic therapy (PDT), we synthesized multifunctional cobalt ferrite (CoFe2O4) nanoparticles (CoFe2O4-HPs-FAs) functionalized by coating them with hematoporphyrin (HP) for introducing photo-functionality and by conjugating with folic acid (FA) for targeting cancer cells. We evaluated the activities of the CoFe2O4-HPs-FAs by checking magnetic resonance imaging (MRI) in vitro, its biocompatibility, and photodynamic anticancer activities on FA receptor (FR)-positive and FR-negative cancer cell lines, Hela, KB, MCF-7, and PC-3 cells, to use for clinical applications. In this study, we have demonstrated that the CoFe2O4-HPs-FAs have good MRI and biocompatibility with non-cytotoxicity, and remarkable photodynamic anticancer activities at very low concentrations regardless of cell types. Particularly, the photo-killing abilities in 3.13 μg/mL of CoFe2O4-HPs-FAs were measured to be 91.8% (p < 0.002) for Hela, 94.5% (p < 0.007) for KB, 79.1% (p < 0.003) for MCF-7, and 71.3% (p < 0.006) for PC-3. The photodynamic anticancer activities in 6.25 and 12.5 μg/mL of CoFe2O4-HPs-FAs were measured to be over 95% (p < 0.004) to almost 100% regardless of cell types. The newly developed multifunctional CoFe2O4-HPs-FAs are effective for PDT and have potential as therapeutic agents for MRI-based PDT, because they have a high saturation value of magnetization and superparamagnetism. |
| Databáze: | OpenAIRE |
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