Regulation of scute function by extramacrochaete in vitro and in vivo
| Autor: | Maria C. Alonso, Hella Huikeshoven, Carlos V. Cabrera |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
animal structures
Molecular Sequence Data Gene Expression Repressor Genes Insect In Vitro Techniques Biology Transcription (biology) Gene expression Morphogenesis Animals Amino Acid Sequence Molecular Biology In Situ Hybridization Genetics Basic helix-loop-helix Helix-Loop-Helix Motifs Wild type Gene Expression Regulation Developmental Sense Organs RNA Immunohistochemistry Cell biology Imaginal disc Drosophila Scute Epitope Mapping Developmental Biology |
| Zdroj: | Development. 120:3595-3603 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.120.12.3595 |
| Popis: | The pattern of adult sensilla in Drosophila is established by the dosage-sensitive interaction of two antagonistic groups of genes. Sensilla development is promoted by members of the achaete-scute complex and the daughterless gene whereas it is suppressed by whereas extramacrochaete (emc) and hairy. All these genes encode helix-loop-helix proteins. The products of the achaete-scute complex and daughterless interact to form heterodimers able to activate transcription. In this report, we show that (1) extra-macrochaete forms heterodimers with the achaete, scute, lethal of scute and daughterless products; (2) extra-macrochaete inhibits DNA-binding of Achaete, Scute and Lethal of Scute/Daughterless heterodimers and Daughter-less homodimers and (3) extramacrochaete inhibits tran-scription activation by heterodimers in a yeast assay system. In addition, we have studied the expression patterns of scute in wild-type and extramacrochaete mutant imaginal discs. Expression of scute RNA during imaginal development occurs in groups of cells, but high levels of protein accumulate in the nuclei of only a subset of the RNA-expressing cells. The pattern is dynamic and results in a small number of protein-containing cells that correspond to sensillum precursors. extramacrochaete loss-of-function alleles develop extra sensilla and correspondingly display a larger number of cells with scute protein. These cells appear to arise from those that in the wild type already express scute RNA; hence, extramacrochaete is a repressor of scute function whose action may take place post-tran-scriptionally. |
| Databáze: | OpenAIRE |
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