Xinfangfangia humi sp. nov., isolated from soil amended with humic acid

Autor: John A. McInroy, Dominique Clermont, Alexis Criscuolo, Stefanie P. Glaeser, Hans-Jürgen Busse, Peter Kämpfer
Přispěvatelé: Justus-Liebig-Universität Gießen = Justus Liebig University (JLU), Veterinärmedizinische Universität Wien, Auburn University (AU), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Collection de l'Institut Pasteur (CIP), Institut Pasteur [Paris] (IP), Justus-Liebig-Universität Gießen (JLU), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
MESH: Sequence Analysis
DNA
Spermidine
Ubiquinone
[SDV]Life Sciences [q-bio]
Diaminopimelic Acid
01 natural sciences
chemistry.chemical_compound
RNA
Ribosomal
16S
MESH: Rhodobacteraceae
Humic acid
MESH: Ubiquinone
Rhodobacteraceae
MESH: Spermidine
MESH: Putrescine
MESH: Phylogeny
Phospholipids
Phylogeny
Soil Microbiology
chemistry.chemical_classification
Base Composition
Phylogenetic tree
Strain (chemistry)
Fatty Acids
General Medicine
Bacterial Typing Techniques
MESH: Fatty Acids
MESH: RNA
Ribosomal
16S
[SDE]Environmental Sciences
Alabama
DNA
Bacterial
Xinfangfangia
MESH: Humic Substances
Diamino acid
Biology
010603 evolutionary biology
Microbiology
MESH: Bacterial Typing Techniques
03 medical and health sciences
MESH: Base Composition
Cadaverine
Botany
Putrescine
MESH: Diaminopimelic Acid
Ecology
Evolution
Behavior and Systematics
Humic Substances
MESH: Cadaverine
MESH: Phospholipids
Xinfangfangia humi
Sequence Analysis
DNA
16S ribosomal RNA
MESH: DNA
Bacterial
MESH: Alabama
030104 developmental biology
chemistry
MESH: Soil Microbiology
Peptidoglycan
Zdroj: International Journal of Systematic and Evolutionary Microbiology
International Journal of Systematic and Evolutionary Microbiology, 2019, 69 (7), pp.2070-2075. ⟨10.1099/ijsem.0.003434⟩
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2019, 69 (7), pp.2070-2075. ⟨10.1099/ijsem.0.003434⟩
ISSN: 1466-5026
1466-5034
Popis: International audience; Two slightly beige-pigmented, Gram-stain-negative, rod-shaped bacterial strains, IMT-291T and IMT-297, were isolated from soil in a field located in Malvern, Alabama, USA. The source soil had been amended with humic acid and continuously used for the cultivation of worms used for fish bait. It is still conceivable that the source of the strains is from the humic acid amendment, although all attempts to isolate the novel phenotypes from the humic acid source have failed. The two strains were identical based on morphology, growth rate and subsequently by 16S rRNA gene sequences, but showed differences in genomic fingerprint patterns generated by rep-PCR. Phylogenetic analysis based on the 16S rRNA gene revealed a placement of the strain in a distinct cluster with Xinfangfangia soli (97.2 % 16S rRNA gene sequence similarity) and in close proximity to the genus Falsirhodobacter with highest 16S rRNA gene sequence similarity of 95.3 % to the type strain of Falsirhodobacter deserti. Sequence similarities to all other type strains were below 95.0 %. The chemotaxonomic analysis showed a clear similarity to the genus Xinfangfangia. The main cellular fatty acids of the strain were C18 : 1 ω7c, 11-methly-C18 : 1 ω7c and C16 : 0. The major quinone was ubiquinone Q-10. Phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine were predominant in the polar lipid profile. The polyamine pattern contained the major compound spermidine and moderate amounts of putrescine and cadaverine. The diamino acid of the peptidoglycan was meso-diaminopimelic acid. Based on phylogenetic, chemotaxonomic and phenotypic analyses we propose a new species of the genus Xinfangfangia, with the name Xinfangfangiahumi sp. nov. and strain IMT-291T (=LMG 30636T=CIP 111625T=CCM 8858T) as type strain.
Databáze: OpenAIRE
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