Graphene oxide-silver nanoparticle hybrid material: an integrated nanosafety study in zebrafish embryos

Autor: Aline Maria Zigiotto de Medeiros, Oswaldo Luiz Alves, Vera Lúcia de Castro, Gabriela H. da Silva, Diego Stéfani T. Martinez, Latif U. Khan, Carlos Ospina
Přispěvatelé: ALINE MARIA ZIGIOTTO DE MEDEIROS, CNPEM, LATIF ULLAH KHAN, CNPEM, GABRIELA HELENA DA SILVA, CNPEM, CARLOS ALBERTO OSPINA, CNPEM, OSWALDO LUIZ ALVES, IQ-UNICAMP, VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA, DIEGO STÉFANI TEODORO MARTINEZ, CNPEM.
Rok vydání: 2020
Předmět:
Embryo
Nonmammalian
Nanomateriais
Health
Toxicology and Mutagenesis
0211 other engineering and technologies
Nanoparticle
Metal Nanoparticles
02 engineering and technology
010501 environmental sciences
Ecotoxicology
01 natural sciences
Silver nanoparticle
Environmental pollution
law.invention
law
GE1-350
Zebrafish
Graphene oxide
Peixe de Água Doce
Chemistry
Silver Compounds
Oxides
General Medicine
Chorion
Alternative methods
Pollution
Membrane
TD172-193.5
Grafeno
Toxicity
embryonic structures
Graphite
Hybrid material
Nanoecotoxicology
Silver
chorion
Animals
Nanotoxicity
Nanomaterials
0105 earth and related environmental sciences
021110 strategic
defence & security studies
Danio rerio
Composto de Carbono
Graphene
Nanohybrids
Public Health
Environmental and Occupational Health
Acute toxicity
Environmental sciences
Nanotoxicology
Toxidez
Biophysics
Water Pollutants
Chemical
Zdroj: Ecotoxicology and Environmental Safety, Vol 209, Iss, Pp 111776-(2021)
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
ISSN: 1090-2414
Popis: This work reports an integrated nanosafety study including the synthesis and characterization of the graphene oxide-silver nanoparticle hybrid material (GO-AgNPs) and its nano-ecotoxicity evaluation in the zebrafish embryo model. The influences of natural organic matter (NOM) and a chorion embryo membrane were considered in this study, looking towards more environmentally realistic scenarios and standardized nanotoxicity testing. The nanohybrid was successfully synthesized using the NaBH4 aqueous method, and AgNPs (~ 5.8 nm) were evenly distributed over the GO surface. GO-AgNPs showed a dose-response acute toxicity: the LC50 was 1.5 mg L-1 for chorionated embryos. The removal of chorion, however, increased this toxic effect by 50%. Furthermore, the presence of NOM mitigated mortality, and LC50 for GO-AgNPs changed respectively from 2.3 to 1.2 mg L-1 for chorionated and de-chorionated embryos. Raman spectroscopy confirmed the ingestion of GO by embryos; but without displaying acute toxicity up to 100 mg L-1, indicating that the silver drove toxicity down. Additionally, it was observed that silver nanoparticle dissolution has a minimal effect on these observed toxicity results. Finally, understanding the influence of chorion membranes and NOM is a critical step towards the standardization of testing for zebrafish embryo toxicity in safety assessments and regulatory issues. Made available in DSpace on 2021-04-05T15:33:57Z (GMT). No. of bitstreams: 1 Castro-Graphene-oxide-2021.pdf: 14395540 bytes, checksum: 7365aff4870953b574a05182a1c75282 (MD5) Previous issue date: 2021
Databáze: OpenAIRE
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