Deleterious effects of gestational diabetes mellitus on the characteristics of the rectus abdominis muscle associated with pregnancy-specific urinary incontinence

Autor: Sérgio Luis Felisbino, Gabriela Marini, Sarah M. B. Costa, Selma Maria Michelin Matheus, João Paulo de Castro Marcondes, Juliana Ferreira Floriano, Ariane Leite Rozza, Raghavendra Lakshmana Shetty Hallur, Joelcio Francisco Abbade, José Eduardo Corrente, Claudia Garcia Magalhães, Fernanda Piculo, Marilza Vieira Cunha Rudge, Sthefanie K. Nunes, Roberto Antonio de Araújo Costa, Iracema de Mattos Paranhos Calderon, David Rafael Abreu Reyes, Giovana Vesentini, Angélica Mércia Pascon Barbosa
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidade Sagrado Coração
Rok vydání: 2020
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1872-8227
Popis: Made available in DSpace on 2020-12-12T02:16:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-08-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Aims: To evaluate the effects of gestational diabetes mellitus (GDM) on the structural characteristics of the rectus abdominis muscle (RAM) and its indirect effects on pregnancy-specific urinary incontinence (PSUI). Methods: A total of 92 pregnant women were divided into four groups, according to their clinical conditions: non-GDM continent, non-GDM associated PSUI, GDM continent and GDM associated PSUI. The muscle morphometry (histochemistry and immunohistochemistry) for the fiber types and collagen fiber distribution, the ultrastructural analysis (transmission electron microscopy), the protein expression of fiber types and calcium signaling (Western blotting), and the content of types I and III collagen fiber (ELISA) in RAM collected at delivery were assessed. Results: The GDM groups presented a significantly increased number of slow fibers and slow-twitch oxidative fiber expression; decreased fiber area, number of fast fibers, and area of collagen; an increase in central nuclei; ultrastructural alterations with focal lesion areas such as myeloid structures, sarcomere disorganization, and mitochondrial alteration. The PSUI groups presented a considerable decrease in types I and III collagen contents and the localization of collagen fiber. Conclusions: Our data reveal that GDM causes morphological, biochemical and physiological changes in the RAM, and this might predispose women to PSUI. Perinatal Diabetes Research Center University Hospital Botucatu Medical School Univ Estadual Paulista_UNESP São Paulo State University (UNESP) Botucatu Medical School Department of Gynecology and Obstetrics São Paulo State University (UNESP) School of Philosophy and Sciences Department of Physical Therapy and Occupational Therapy São Paulo State University (UNESP) Institute of Biosciences Department of Morphology Universidade Sagrado Coração Department of Health Sciences São Paulo State University (UNESP) Institute of Biosciences Biostatistics Department São Paulo State University (UNESP) Institute of Biosciences Department of Anatomy Perinatal Diabetes Research Center University Hospital Botucatu Medical School Univ Estadual Paulista_UNESP São Paulo State University (UNESP) Botucatu Medical School Department of Gynecology and Obstetrics São Paulo State University (UNESP) School of Philosophy and Sciences Department of Physical Therapy and Occupational Therapy São Paulo State University (UNESP) Institute of Biosciences Department of Morphology São Paulo State University (UNESP) Institute of Biosciences Biostatistics Department São Paulo State University (UNESP) Institute of Biosciences Department of Anatomy FAPESP: 2014/26852-6 FAPESP: 2016/09710-9
Databáze: OpenAIRE
Externí odkaz: