Inhibition and Inactivation of Uropathogenic Escherichia coli Biofilms on Urinary Catheters by Sodium Selenite
| Autor: | Meera Surendran Nair, Amoolya Narayanan, Mary Anne Roshni Amalaradjou, Muhammed Shafeekh Muyyarikkandy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.drug_class Urinary system 030106 microbiology Biofilm inhibition Antibiotics chemistry.chemical_element medicine.disease_cause urologic and male genital diseases Catalysis biofilm Microbiology lcsh:Chemistry Inorganic Chemistry urinary catheters 03 medical and health sciences medicine Physical and Theoretical Chemistry selenium lcsh:QH301-705.5 Molecular Biology Inhibitory effect Incubation Escherichia coli Spectroscopy urogenital system Organic Chemistry Biofilm General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses female genital diseases and pregnancy complications Computer Science Applications 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry gene expression exopolysaccharide UPEC Selenium |
| Zdroj: | International Journal of Molecular Sciences Volume 19 Issue 6 International Journal of Molecular Sciences, Vol 19, Iss 6, p 1703 (2018) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms19061703 |
| Popis: | Urinary tract infections (UTI) are the most common hospital-acquired infections in humans and are caused primarily by uropathogenic Escherichia coli (UPEC). Indwelling urinary catheters become encrusted with UPEC biofilms that are resistant to common antibiotics, resulting in chronic infections. Therefore, it is important to control UPEC biofilms on catheters to reduce the risk for UTIs. This study investigated the efficacy of selenium for inhibiting and inactivating UPEC biofilms on urinary catheters. Urinary catheters were inoculated with UPEC and treated with 0 and 35 mM selenium at 37 ° C for 5 days for the biofilm inhibition assay. In addition, catheters with preformed UPEC biofilms were treated with 0, 45, 60, and 85 mM selenium and incubated at 37 ° C. Biofilm-associated UPEC counts on catheters were enumerated on days 0, 1, 3, and 5 of incubation. Additionally, the effect of selenium on exopolysacchride (EPS) production and expression of UPEC biofilm-associated genes was evaluated. Selenium at 35 mM concentration was effective in preventing UPEC biofilm formation on catheters compared to controls (p < 0.05). Further, this inhibitory effect was associated with a reduction in EPS production and UPEC gene expression. Moreover, at higher concentrations, selenium was effective in inactivating preformed UPEC biofilms on catheters as early as day 3 of incubation. Results suggest that selenium could be potentially used in the control of UPEC biofilms on urinary catheters. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |