CD43 Deficiency Has No Impact in Competitive In Vivo Assays of Neutrophil or Activated T Cell Recruitment Efficiency
| Autor: | Douglas A. Carlow, Hermann J. Ziltener |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Neutrophils
Sialoglycoproteins T-Lymphocytes T cell Immunology Succinimides Dermatitis Inflammation Context (language use) Peritonitis Biology Lymphocyte Activation N-Acetylglucosaminyltransferases Mice Venules Peritoneum immune system diseases In vivo hemic and lymphatic diseases medicine Animals Immunology and Allergy Leukocyte Rolling Skin CD43 Leukosialin hemic and immune systems Fluoresceins Receptors Fibroblast Growth Factor Molecular biology Mice Mutant Strains Extravasation In vitro Mice Inbred C57BL medicine.anatomical_structure Biological Assay medicine.symptom |
| Zdroj: | The Journal of Immunology. 177:6450-6459 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.177.9.6450 |
| Popis: | Using noncompetitive methodologies comparing CD43+/+ and CD43−/− mice, it has been reported that CD43−/− leukocytes exhibit reduced recruitment efficiency to sites of inflammation. More recent analyses demonstrate that CD43 on activated T cells can function as an E-selectin ligand (E-SelL) in vitro, suggesting that CD43 might promote rolling interactions during recruitment of leukocytes and account for the reported recruitment deficits in CD43−/− T cells and neutrophils in vivo. Internally controlled competitive in vivo methods using fluorescent tracking dyes were applied to compare recruitment efficiency of CD43+/+ vs CD43−/− activated T cells to inflamed skin and of peripheral blood neutrophils to inflamed peritoneum. A simple CFSE perfusion method was developed to distinguish arterial/venous vasculature and confirm appropriate extravasation through venules in a Con A-induced cutaneous inflammation model. In vivo recruitment of peripheral blood neutrophils to inflamed peritoneum was core 2 GlcNAcT-I dependent, but recruitment efficiency was not influenced by absence of CD43. There were also no significant differences in core 2 GlcNAcT-I-dependent, selectin-dependent, cutaneous recruitment of activated T cells from CD43+/+ and congenic CD43−/− mice in either B6 or P-selectin−/− recipients despite biochemical confirmation that a CD43-specific E-SelL was present on activated T cells. We conclude that recruitment of neutrophils and activated T cells in these in vivo models is not influenced by CD43 expression and that if CD43 on activated T cells performs an E-SelL function in vivo, it contributes in a limited physiological context. |
| Databáze: | OpenAIRE |
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