A RT-PCR assay for the detection of encephalomycarditis virus infections in pigs
| Autor: | Heidy Díaz de Arce, Lester J. Pérez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
pig Strain (chemistry) viruses lcsh:QR1-502 RNA Biology Microbiology Virology Genome Virus lcsh:Microbiology Reverse transcription polymerase chain reaction chemistry.chemical_compound Real-time polymerase chain reaction chemistry RT-PCR assay Veterinarian Microbiology Encephalomyocarditis virus Glycoprotein DNA Research Paper |
| Zdroj: | Brazilian Journal of Microbiology, Vol 40, Iss 4, Pp 988-993 (2009) Brazilian Journal of Microbiology v.40 n.4 2009 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Volume: 40, Issue: 4, Pages: 988-993, Published: DEC 2009 |
| ISSN: | 1678-4405 1517-8382 |
| Popis: | Encephalomyocarditis virus (EMCV) infections can cause losses in pig farms all over the world. Rapid, sensitive and unequivocal detection of this virus is therefore essential for the diagnosis and control of the disease. An RT-PCR assay was developed, optimized and evaluated for encephalomyocarditis virus detection in organ based on a pair of primers that amplifies a 165 bp DNA fragment from a highly conserved nucleotide region of the viral 3D glycoprotein. PCR products of the expected size were obtained from Cuban EMCV 744/03 strain. Non-specific reactions were not observed when other porcine RNA genome viruses and uninfected cells were used. The analytical sensitivity of the test was estimated to be 2 TCID50/50 μL. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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