GABAergic Neuron-Specific Loss of Ube3a Causes Angelman Syndrome-Like EEG Abnormalities and Enhances Seizure Susceptibility
| Autor: | Ian F. G. King, Michael L. Wallace, Michael S. Sidorov, Benjamin D. Philpot, Mark J. Zylka, Ype Elgersma, Bin Gu, Geeske M. van Woerden, Richard J. Weinberg, Alain C. Burette, Ji Eun E. Han, Matthew C. Judson |
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| Přispěvatelé: | Neurosciences |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
congenital hereditary and neonatal diseases and abnormalities Neocortex medicine.diagnostic_test General Neuroscience Electroencephalography Biology medicine.disease 03 medical and health sciences Epilepsy Glutamatergic 030104 developmental biology 0302 clinical medicine Neurodevelopmental disorder medicine.anatomical_structure Angelman syndrome UBE3A medicine GABAergic Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Neuron, 90(1), 56-69. Cell Press |
| ISSN: | 1097-4199 0896-6273 |
| Popis: | Loss of maternal UBE3A causes Angelman syndrome (AS), a neurodevelopmental disorder associated with severe epilepsy. We previously implicated GABAergic deficits onto layer (L) 2/3 pyramidal neurons in the pathogenesis of neocortical hyperexcitability, and perhaps epilepsy, in AS model mice. Here we investigate consequences of selective Ube3a loss from either GABAergic or glutamatergic neurons, focusing on the development of hyperexcitability within L2/3 neocortex and in broader circuit and behavioral contexts. We find that GABAergic Ube3a loss causes AS-like increases in neocortical EEG delta power, enhances seizure susceptibility, and leads to presynaptic accumulation of clathrin-coated vesicles (CCVs) – all without decreasing GABAergic inhibition onto L2/3 pyramidal neurons. Conversely, glutamatergic Ube3a loss fails to yield EEG abnormalities, seizures, or associated CCV phenotypes, despite impairing tonic inhibition onto L2/3 pyramidal neurons. These results substantiate GABAergic Ube3a loss as the principal cause of circuit hyperexcitability in AS mice, lending insight into ictogenic mechanisms in AS. |
| Databáze: | OpenAIRE |
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