The In Vitro Effect of Prostaglandin E2 and F2α on the Chemerin System in the Porcine Endometrium during Gestation
| Autor: | Grzegorz Kopij, Nina Smolinska, Karolina Szymanska, Marlena Gudelska, Edyta Rytelewska, Kamil Dobrzyn, Barbara Kaminska, Marta Kiezun, Katarzyna Kisielewska, Tadeusz Kaminski, Ewa Zaobidna, Kinga Bors |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Chemokine Swine Endometrium Dinoprost lcsh:Chemistry 0302 clinical medicine Pregnancy Receptor lcsh:QH301-705.5 Spectroscopy early pregnancy biology chemerin receptors General Medicine Computer Science Applications medicine.anatomical_structure PGF2α porcine uterus Female Receptors Chemokine PGE2 Chemokines chemerin medicine.medical_specialty Adipokine 030209 endocrinology & metabolism CMKLR1 Catalysis Article Dinoprostone GPR1 Inorganic Chemistry 03 medical and health sciences Internal medicine medicine Chemerin Animals Physical and Theoretical Chemistry Molecular Biology Organic Chemistry 030104 developmental biology Endocrinology lcsh:Biology (General) lcsh:QD1-999 biology.protein Hormone |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 15 International Journal of Molecular Sciences, Vol 21, Iss 5213, p 5213 (2020) |
| ISSN: | 1422-0067 |
| Popis: | Chemerin belongs to the group of adipocyte-derived hormones known as adipokines, which are responsible mainly for the control of energy homeostasis. Adipokine exerts its influence through three receptors: Chemokine-like receptor 1 (CMKLR1), G protein-coupled receptor 1 (GPR1), and C-C motif chemokine receptor-like 2 (CCRL2). A growing body of evidence indicates that chemerin participates in the regulation of the female reproductive system. According to the literature, the expression of chemerin and its receptors in reproductive structures depends on the local hormonal milieu. The aim of this study was to investigate the in vitro effect of prostaglandins E2 (PGE2) and F2&alpha (PGF2&alpha ) on chemerin and chemerin receptor (chemerin system) mRNAs (qPCR) and proteins (ELISA, Western blotting) in endometrial tissue explants collected from early-pregnant gilts. Both PGE2 and PGF2&alpha significantly influenced the expression of the chemerin gene, hormone secretion, and the expression of chemerin receptor genes and proteins. The influence of both prostaglandins on the expression of the chemerin system varied between different stages of gestation. This is the first study to describe the modulatory effect of PGE2 and PGF2&alpha on the expression of the chemerin system in the porcine uterus during early gestation. |
| Databáze: | OpenAIRE |
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