Detection and neutralization of venom by ovine antiserum in experimental envenoming by Bothrops jararaca
| Autor: | Alexandrina Sartori, J. Ferreira, M. F. Bastos, C. M. Peres |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Bothrops jararaca
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Venom Spleen ovine anti-bothropic serum Urine Pharmacology Toxicology Ovis complex mixtures Neutralization In vivo lcsh:RA1190-1270 lcsh:Zoology medicine Ovine antibothropic serum Animalia lcsh:QL1-991 Envenomation lcsh:Toxicology. Poisons Antiserum venom neutralization biology biology.organism_classification Infectious Diseases medicine.anatomical_structure Immunology Animal Science and Zoology Parasitology enzyme-linked immunosorbent assay |
| Zdroj: | Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 12, Issue: 1, Pages: 124-136, Published: 2006 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 12, Iss 1, Pp 124-136 (2006) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP ResearcherID Journal of Venomous Animals and Toxins including Tropical Diseases v.12 n.1 2006 The Journal of venomous animals and toxins including tropical diseases |
| Popis: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:21:51Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:43:47Z : No. of bitstreams: 1 2-s2.0-33645847565.pdf: 112561 bytes, checksum: 7e7d8abd990f4f1cb8d35fdac154c498 (MD5) Made available in DSpace on 2014-05-27T11:21:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-04-26 In this study we optimized an enzyme-linked immunosorbent assay (ELISA) to evaluate bothropic venom levels in biological samples. These samples were obtained by two distinct protocols. In the first one, Swiss mice were injected with 1 LD 50 of Bothrops jararaca (B. jararaca) venom and 15 minutes later, animals were treated with ovine antibothropic serum. Blood and spleen homogenate samples were obtained 6 hours after antiserum therapy. Ovine antibothropic serum significantly neutralized venom levels in serum and spleen. In the second protocol, BALB/c mice were injected with 1 LD 50 of bothropic venom by either intraperitoneal (IP) or intradermal (ID) route and venom levels were evaluated 1, 3 and 6 hours after, in blood, spleen homogenates and urine. Serum and splenic venom levels were significantly higher in animals envenomed by IP route comparing with animals envenomed by ID route. Higher venom levels were also detected in urine samples from animals envenomed by IP route. However, these differences were not statistically significant. These results demonstrated that the optimized ELISA was adequate to quantify venom levels in different biological samples. This assay could, therefore, substitute the in vivo neutralizing assay and also be useful to evaluate the severity of human and experimental envenomations. Department of Microbiology and Immunology Institute of Biosciences São Paulo State University, Botucatu, São Paulo São Carlos Institute of Physics University of São Paulo, USP, São Paulo Departamento de Microbiologia e Imunologia Instituto de Biociências UNESP, Botucatu, São Paulo, 18618-000 Department of Microbiology and Immunology Institute of Biosciences São Paulo State University, Botucatu, São Paulo Departamento de Microbiologia e Imunologia Instituto de Biociências UNESP, Botucatu, São Paulo, 18618-000 |
| Databáze: | OpenAIRE |
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