Standardization of retention time data for AMT tag proteomics database generation
| Autor: | Irina A. Tarasova, V. V. Evreinov, Marina L. Pridatchenko, A. V. Gorshkov, Christophe Masselon, Mikhail V. Gorshkov, Sylvie Kieffer-Jaquinod, Vilém Guryča |
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| Rok vydání: | 2009 |
| Předmět: |
Proteomics
Chromatography Database Standardization Scale (ratio) Chemistry Molecular Sequence Data Clinical Biochemistry Proteins Context (language use) Cell Biology General Medicine Reversed-phase chromatography computer.software_genre Mass spectrometry Biochemistry Mass Spectrometry Analytical Chemistry Identification (information) Proteome Amino Acid Sequence Databases Protein computer Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B. 877:433-440 |
| ISSN: | 1570-0232 |
| Popis: | The combination of liquid chromatography (LC) with mass spectrometry (MS) has become a mainstream proteome analysis strategy. In LC-MS, measured masses possess their "universal" scale derived from atomic mass tables. In contrast, the observed LC retention times (RT) are not tied to a conventional time scale, and depend on experimental conditions. However, RT data, being explicitly orthogonal to MS, offer relevant information for proteome characterization. We present here a strategy for peptides RT data standardization, based on the generation of a standard scale using retention prediction models, which enables sharing of identification databases in the context of multi-laboratory research. |
| Databáze: | OpenAIRE |
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