Surgery Induces Human Mononuclear Cell Arginase I Expression
| Autor: | Juan B. Ochoa, Andrew C. Bernard, Bernard R. Boulanger, Paul A. Kearney, Matthew D. Shane, Mary E. Maley, Betty J. Tsuei, Lawrence A. Shirley |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Male medicine.medical_specialty Arginine medicine.medical_treatment Population Nitric Oxide Critical Care and Intensive Care Medicine Peripheral blood mononuclear cell Nitric oxide chemistry.chemical_compound Immune system medicine Humans education Aged Aged 80 and over Analysis of Variance education.field_of_study Arginase Interleukin-6 business.industry Interleukin Middle Aged Interleukin-10 Surgery Cytokine Liver chemistry Case-Control Studies Immune System Surgical Procedures Operative Leukocytes Mononuclear Female business |
| Zdroj: | Journal of Trauma and Acute Care Surgery. 51:497-502 |
| ISSN: | 0022-5282 |
| DOI: | 10.1097/00005373-200109000-00012 |
| Popis: | Background: Arginase is a metabolic enzyme for the amino acid arginine that participates in the immune response to trauma. We hypothesize that surgical trauma induces arginase expression and activity in the human immune system. Methods: Peripheral mononuclear cell (MNC) arginase activity and expression and plasma nitric oxide metabolites and interleukin (IL)-10 were measured in patients undergoing elective general surgery. Twenty-two healthy volunteers served as a comparison population. Results: MNC arginase activity increased within 6 hours of surgery (p < 0.05) and coincided with increased arginase I protein expression. Plasma nitric oxide metabolites decreased significantly postoperatively (p < 0.05). Patients lacking an elevation in IL-10 failed to demonstrate increased MNC arginase activity. Conclusion: Increased MNC arginase expression may contribute to post-surgical immune dysfunction by affecting arginine use and availability and nitric oxide metabolism in the immune system. Plasma IL-10 may play a role in regulating MNC arginase activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|