Role of the Activation of the Nuclear Enzyme Poly(ADP-Ribose) Polymerase in the Pathogenesis of Periodontitis
| Autor: | Jon G. Mabley, Anita Marton, Csaba Szabó, Katalin Komjáti, Erzsébet Fehér, Zsolt Lohinai |
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| Rok vydání: | 2003 |
| Předmět: |
Male
0301 basic medicine Poly Adenosine Diphosphate Ribose Poly ADP ribose polymerase Alveolar Bone Loss Gingiva Connective tissue Inflammation Nitric oxide Mice Plasma 03 medical and health sciences Enzyme activator chemistry.chemical_compound 0302 clinical medicine medicine Animals Rats Wistar Periodontitis Ligation General Dentistry Evans Blue Cell Nucleus Mice Knockout Chemistry Mouth Mucosa Nuclear Proteins 030206 dentistry Phenanthrenes medicine.disease Molecular biology Extravasation Rats Enzyme Activation Disease Models Animal 030104 developmental biology medicine.anatomical_structure Connective Tissue medicine.symptom |
| Zdroj: | Journal of Dental Research. 82:987-992 |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/154405910308201210 |
| Popis: | We have investigated the role of the activation of nuclear poly(ADP-ribose) polymerase (PARP) enzyme, a mediator of downstream nitric oxide toxicity, using a combined approach of pharmacological inhibition and genetic disruption in a ligature-induced-periodontitis model in rats and mice. Immunohistochemical analysis revealed significantly increased poly(ADP-ribose) nuclear staining (indicative of PARP activation) in the subepithelial connective tissue of the ligated side compared with the non-ligated side. Ligation-induced periodontitis resulted in marked plasma extravasation in the gingivomucosal tissue and led to alveolar bone destruction compared with the non-ligated side, as measured by the Evans blue technique and by videomicroscopy, respectively. PARP inhibition with PJ34, as well as genetic PARP-1 deficiency, significantly reduced the extravasation and the alveolar bone resorption of the ligated side compared with controls. Thus, PARP activation contributes to the development of periodontal injury. Inhibition of PARP may represent a novel host response modulatory approach for the therapy of periodontitis. |
| Databáze: | OpenAIRE |
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