Effect of photodynamic therapy in combination with various irrigation protocols on an endodontic multispecies biofilm ex vivo
| Autor: | Daniela Hoedke, C. Enseleit, Sebastian Schlafer, D. Gruner, Kerstin Bitter, Henrik Dommisch, Irene Dige |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030103 biophysics Veterinary medicine medicine.medical_treatment Dentistry Photodynamic therapy Enterococcus faecalis 03 medical and health sciences chemistry.chemical_compound Random Allocation 0302 clinical medicine medicine Humans Therapeutic Irrigation General Dentistry Antibacterial photodynamic therapy biology Irrigation protocol Root Canal Irrigants Chemistry business.industry Chlorhexidine Biofilm Prevotella intermedia 030206 dentistry biology.organism_classification Combined Modality Therapy Bacterial Load Streptococcus oralis Photochemotherapy Sodium hypochlorite Biofilms Multispecies biofilm Dental Pulp Cavity business Root canal disinfection Bacteria medicine.drug |
| Zdroj: | Hoedke, D, Enseleit, C, Gruner, D, Dommisch, H, Schlafer, S, Dige, I & Bitter, K 2018, ' Effect of photodynamic therapy in combination with various irrigation protocols on an endodontic multispecies biofilm ex vivo ', International Endodontic Journal, vol. 51, no. S1, pp. e23-e34 . https://doi.org/10.1111/iej.12763 |
| ISSN: | 1365-2591 |
| DOI: | 10.1111/iej.12763 |
| Popis: | Aim: To analyse the antibacterial effect of photodynamic therapy (PDT) in combination with various irrigation protocols on a multispecies biofilm in root canals ex vivo. Methodology: A total of 160 extracted human single-rooted teeth were divided into four groups (n = 40). In group G1, root canals were instrumented up to size 60 (control group), whereas in G2 to G4 canals were enlarged up to size 40. All root canals were inoculated with a multispecies biofilm (Enterococcus faecalis, Streptococcus oralis, Prevotella intermedia) for 5 days. In G2 to G4, instrumentation up to size 60 was performed using 0.9% sodium chloride (NaCl) (G2), 1% sodium hypochlorite (NaOCl) (G3), 1% NaOCl and a final irrigation with 2% chlorhexidine (CHX) (G4), respectively. In all groups half of the specimens received adjunctive PDT using phenothiazine chloride as photosensitizer and a diode laser (wavelength 660 nm). Counts of colony-forming units (CFUs) in each group were analysed separately for planktonic and dentine-adherent bacteria immediately after therapy (T1) and after 5 days of further incubation (T2). Descriptive statistics and two-way analysis of variance were carried out to analyse reduction of planktonic bacteria and nonparametric tests were used to analyse dentine-adherent bacteria. Results: CFU reduction in planktonic bacteria was significantly affected by the irrigation protocol at T1 and T2 (P < 0.0001), but PDT significantly reduced CFUs only at T2 (P = 0.01; anova). Irrigation using NaOCl, CHX and adjunctive PDT significantly reduced CFUs at T2 (P < 0.0001; Tukey HSD) compared to the control group. In 85.6% of all samples the same categories of CFU counts in both planktonic and dentine-adherent bacteria were detected at T1 and T2. Conclusions: Adjunctive photodynamic therapy in combination with an irrigation protocol including NaOCl and CHX was an effective method for reduction of bacterial biofilm inside the root canals of extracted teeth. |
| Databáze: | OpenAIRE |
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