Comparison of behavioral assays for assessing toxicant-induced alterations in neurological function in larval fathead minnows
| Autor: | Julie C. Krzykwa, Marlo K. Sellin Jeffries |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Environmental Engineering
Health Toxicology and Mutagenesis 0208 environmental biotechnology Neurological function Cyprinidae Physiology 02 engineering and technology 010501 environmental sciences 01 natural sciences Hazardous Substances chemistry.chemical_compound biology.animal Toxicity Tests Animals Environmental Chemistry Organism 0105 earth and related environmental sciences Larva Behavior Animal biology Public Health Environmental and Occupational Health General Medicine General Chemistry Minnow Pollution 020801 environmental engineering chemistry Chlorpyrifos Toxicity Optomotor response Biological Assay Water Pollutants Chemical Toxicant |
| Zdroj: | Chemosphere. 257:126825 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2020.126825 |
| Popis: | Neuroactive compounds are routinely detected in surface waters at concentrations that pose potential threats to wildlife. Exposure to neurotoxicants can adversely affect exposed organism by altering ecologically-important behaviors (e.g., feeding and predator response) that are likely to have important repercussions for populations. These compounds can elicit behavioral effects at concentrations lower than those that induce overt toxicity as indicated by mortality or decreased growth. Though a wide variety of methods have been employed to assess the behavior of early life stage fish, it is unclear which assays are best suited for identifying ecologically-relevant behavioral changes following exposures to neurotoxicants. The goal of the present study was to promote the use of behavioral assays for assessing the behavioral impacts of exposure to neurotoxic compounds by comparing the performance of different behavioral assays in larval fish. To achieve this goal, the sensitivity and practicality of three behavioral assays (i.e., feeding, optomotor response, and C-start assays) were compared in larval fathead minnows exposed to a known neurotoxicant, chlorpyrifos. There were significant alterations in the performance of fathead minnow larvae in all three behavioral assays in response to a 12-d embryo-larval exposure to chlorpyrifos. However, feeding and C-start were the most practical of the selected assays, as they took less time and allowed for larger samples sizes. Further work to standardize behavioral testing methods, and to link alterations to ecologically-relevant behaviors, will help promote the use of these assays when investigating the potential environmental impacts of neurotoxic compounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect