Uncovering mechanisms of rubber biosynthesis in Taraxacum koksaghyz - role of cis-prenyltransferase-like 1 protein
| Autor: | Iris Finkemeier, Christian Schulze Gronover, Ines Lassowskat, Eva Niephaus, Nicole van Deenen, Dirk Prüfer, Boje Müller, Martin Bonin |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Proteomics Latex Proteome Taraxacum Protein subunit Prenyltransferase Dandelion Plant Science Biology complex mixtures 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Dolichol Hemiterpenes Biosynthesis Natural rubber Transferases Genetics Butadienes Plant Proteins chemistry.chemical_classification technology industry and agriculture Inulin Cell Biology Polymer Terpenoid Carbon Triterpenes body regions 030104 developmental biology chemistry Biochemistry visual_art Gene Knockdown Techniques visual_art.visual_art_medium psychological phenomena and processes 010606 plant biology & botany |
| Zdroj: | The Plant journal : for cell and molecular biologyReferences. 100(3) |
| ISSN: | 1365-313X |
| Popis: | The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high-molecular-weight rubber in its roots. The characterization of factors that participate in natural rubber biosynthesis is fundamental for the establishment of T. koksaghyz as a rubber crop. The cis-1,4-isoprene polymers are stored in rubber particles. Located at the particle surface, the rubber transferase complex, member of the cis-prenyltransferase (cisPT) enzyme family, catalyzes the elongation of the rubber chains. An active rubber transferase heteromer requires a cisPT subunit (CPT) as well as a CPT-like subunit (CPTL), of which T. koksaghyz has two homologous forms: TkCPTL1 and TkCPTL2, which potentially associate with the rubber transferase complex. Knockdown of TkCPTL1, which is predominantly expressed in latex, led to abolished poly(cis-1,4-isoprene) synthesis but unaffected dolichol content, whereas levels of triterpenes and inulin were elevated in roots. Analyses of latex from these TkCPTL1-RNAi plants revealed particles that were similar to native rubber particles regarding their particle size, phospholipid composition, and presence of small rubber particle proteins (SRPPs). We found that the particles encapsulated triterpenes in a phospholipid shell stabilized by SRPPs. Conversely, downregulating the low-expressed TkCPTL2 showed no altered phenotype, suggesting its protein function is redundant in T. koksaghyz. MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wild-types discovered putative factors that convert metabolites in biosynthetic pathways connected to isoprenoids or that synthesize components of the rubber particle shell. |
| Databáze: | OpenAIRE |
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