Quantitative analysis of heparanase messenger RNA expression in hepatocellular carcinoma
| Autor: | Nobuaki Kaibara, Masahide Ikeguchi, Tsuyoshi Ueta, Yoshiaki Yamane, Yasuaki Hirooka |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Carcinoma Hepatocellular Cellular differentiation Gene expression medicine Extracellular Humans Heparanase RNA Messenger Aged Glucuronidase Aged 80 and over Messenger RNA business.industry Reverse Transcriptase Polymerase Chain Reaction Liver Neoplasms General Medicine Middle Aged medicine.disease Prognosis Reverse transcription polymerase chain reaction Oncology Hepatocellular carcinoma Cancer cell Surgery Female business |
| Zdroj: | Journal of surgical oncology. 81(3) |
| ISSN: | 0022-4790 |
| Popis: | Background and Objectives In the process of tumor invasion, cancer cells secrete enzymes including heparanase that degrade extracellular matrices and basement membranes of blood vessels. In the present study, the clinical importance of enhanced expression of heparanase messenger RNA (mRNA) in hepatocellular carcinoma (HCC) was evaluated. Methods Fresh tumor and noncancerous liver tissue adjacent to a tumor were obtained from 50 patients with HCC immediately after hepatic resection. The expression levels of heparanase mRNA were analyzed quantitatively, using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR) method, and were compared with clinicopathological findings. Results Relative heparanase mRNA expression level in HCC was significantly lower than that of noncancerous liver tissue (P |
| Databáze: | OpenAIRE |
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