Structure, organization and nucleotide diversity of the mitochondrial control region and cytochrome b of southern water vole (Arvicola sapidus)
| Autor: | Alejandro Centeno-Cuadros, José A. Godoy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Molecular Sequence Data
Cytochrome b Rodentia DNA Mitochondrial Nucleotide diversity Genetics Animals Water vole D-loop Molecular Biology mtDNA control region Genetic diversity extended termination-associated sequences biology Base Sequence Arvicolinae European water vole Cytochromes b biology.organism_classification Locus Control Region Hypervariable region Muridae Evolutionary biology Arvicola Conserved sequence blocks |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | The southern water vole (Arvicola sapidus Miller, 1908) is an endangered rodent whose conservation guidelines should preserve the current genetic variability. We analyze the structure and organization of the mitochondrial control region (CR) in A. sapidus. The CR of this species is characterized by a low guanine-cytosine content, the absence of any repetitive motif within the two hypervariable regions, and the presence of the two extended termination-associated sequences and conserved sequence blocks. Nucleotide diversity comparisons between A. sapidus and the European water vole (Arvicola terrestris) revealed differences in the distribution of genetic variation. Furthermore, we provide primers for the amplification of short and highly polymorphic fragments of CR and cytochrome b especially designed for degraded materials. These markers offer molecular tools to assist in the establishment of future conservation and management guidelines, and will also facilitate studies at different spatial and evolutionary scales of this species. |
| Databáze: | OpenAIRE |
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