Definition and Quantification of Three-Dimensional Imaging Targets to Phenotype Pre-Eclampsia Subtypes: An Exploratory Study

Autor:	Sammy Hermans, Jacob Pilon, Dennis Eschweiler, Johannes Stegmaier, Carmen A. H. Severens–Rijvers, Salwan Al-Nasiry, Marc van Zandvoort, Dimitrios Kapsokalyvas
Přispěvatelé:	RS: GROW - R2 - Basic and Translational Cancer Biology, Pathologie, MUMC+: DA Pat Pathologie (9), RS: GROW - R4 - Reproductive and Perinatal Medicine, Obstetrie & Gynaecologie, MUMC+: MA Medische Staf Obstetrie Gynaecologie (9), RS: MHeNs - R3 - Neuroscience, Genetica & Celbiologie, RS: Carim - B06 Imaging, RS: GROW - School for Oncology and Reproduction
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	pre-eclampsia CLINICAL PRESENTATIONS placenta HUMAN PLACENTA Organic Chemistry General Medicine Catalysis quantification Computer Science Applications APOPTOSIS Inorganic Chemistry HUMAN FETOPLACENTAL VASCULOGENESIS multiphoton microscopy PREGNANCIES NORMAL TERM GROWTH Physical and Theoretical Chemistry MEMBRANE Molecular Biology Spectroscopy SYNCYTIAL KNOTS VILLOUS TROPHOBLAST
Zdroj:	International Journal of Molecular Sciences Volume 24 Issue 4 Pages: 3240 International Journal of Molecular Sciences, 24(4):3240. Multidisciplinary Digital Publishing Institute (MDPI)
ISSN:	1422-0067 1661-6596
DOI:	10.3390/ijms24043240
Popis:	Pre-eclampsia is a severe placenta-related complication of pregnancy with limited early diagnostic and therapeutic options. Aetiological knowledge is controversial, and there is no universal consensus on what constitutes the early and late phenotypes of pre-eclampsia. Phenotyping of native placental three-dimensional (3D) morphology offers a novel approach to improve our understanding of the structural placental abnormalities in pre-eclampsia. Healthy and pre-eclamptic placental tissues were imaged with multiphoton microscopy (MPM). Imaging based on inherent signal (collagen, and cytoplasm) and fluorescent staining (nuclei, and blood vessels) enabled the visualization of placental villous tissue with subcellular resolution. Images were analysed with a combination of open source (FIJI, VMTK, Stardist, MATLAB, DBSCAN), and commercially (MATLAB) available software. Trophoblast organization, 3D-villous tree structure, syncytial knots, fibrosis, and 3D-vascular networks were identified as quantifiable imaging targets. Preliminary data indicate increased syncytial knot density with characteristic elongated shape, higher occurrence of paddle-like villous sprouts, abnormal villous volume-to-surface ratio, and decreased vascular density in pre-eclampsia compared to control placentas. The preliminary data presented indicate the potential of quantifying 3D microscopic images for identifying different morphological features and phenotyping pre-eclampsia in placental villous tissue.
Databáze:	OpenAIRE
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