Site-specific DNA cleavage by Cu(II) complexes of podophyllotoxin derivatives
| Autor: | Hiromu Sakurai, Atsuko Yamashita, Riichi Tawa, Kuo Hsiung Lee, Yasuhiro Imakura |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Stereochemistry
Guanine Molecular Sequence Data DNA Single-Stranded chemistry.chemical_element DNA-Directed DNA Polymerase Biochemistry chemistry.chemical_compound symbols.namesake Gallic Acid medicine Podophyllotoxin Pharmacology Sanger sequencing Base Sequence Syringic acid Copper chemistry Mechanism of action Mutagenesis Site-Directed symbols Spectrophotometry Ultraviolet Hydroxyl radical medicine.symptom DNA Cytosine DNA Damage |
| Zdroj: | Biochemical Pharmacology. 47:1920-1925 |
| ISSN: | 0006-2952 |
| Popis: | Site-specific DNA cleavage in the presence of Cu(II) complexes of podophyllotoxin derivatives was investigated with a modified Sanger sequencing method. Cu(II) complexes of 4'-demethylepipodophyllotoxin (DEPD) and syringic acid (SA) cleaved M13mp18 single-strand DNA site-specifically at both cytosine (C) and guanine (G) positions in the GC rich regions and C position, respectively, at pH 7.8. The apparent binding constants of calf thymus DNA-Cu(II) complexes estimated by the differential UV-absorption spectra revealed that both Cu(II)-VP-16 and -DEPD complexes bind to DNA more strongly than does the Cu(II)-SA complex. |
| Databáze: | OpenAIRE |
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