In vivo digestion of infant formula in piglets: protein digestion kinetics and release of bioactive peptides

Autor: Julien Jardin, Karima Bouzerzour, Isabelle Cuinet, Didier Dupont, Cécile Bonhomme, Isabelle Le Huërou-Luron, François Morgan
Přispěvatelé: Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Alimentation Adaptations Digestives, Nerveuse et Comportementales (ADNC), Institut National de la Recherche Agronomique (INRA), LACTALIS RECHERCHE ET DEVELOPPEMENT (LACTALIS R&D), Groupe Lactalis, Lactalis Nutrition, Lactalis, Nutrition, Métabolismes et Cancer (NuMeCan), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), UR 1341, ADNC, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
030309 nutrition & dietetics
Sus scrofa
Medicine (miscellaneous)
Lactoglobulins
digestion
01 natural sciences
Jejunum
Casein
animal modèle
enfant
formule
2. Zero hunger
0303 health sciences
milk
Nutrition and Dietetics
caseine
Stomach
digestive
oral
and skin physiology
Caseins
Milk Proteins
Infant Formula
peptide
medicine.anatomical_structure
test elisa
Biochemistry
Models
Animal
Electrophoresis
Polyacrylamide Gel
Digestion
Protein digestion
Immunoblotting
Ileum
Biology
03 medical and health sciences
microbiote
medicine
Animals
Humans
animal model
010401 analytical chemistry
cinetique
Infant
Newborn
lait
Small intestine
0104 chemical sciences
Gastrointestinal Tract
Kinetics
Animals
Newborn
Infant formula
Gastric Mucosa
modèle animale
Proteolysis
Lactalbumin
Peptides
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Zdroj: British Journal of Nutrition
British Journal of Nutrition, Cambridge University Press (CUP), 2012, 108 (12), pp.2105-2114. ⟨10.1017/S000711451200027X⟩
British Journal of Nutrition, 2012, 108 (12), pp.2105-2114. ⟨10.1017/S000711451200027X⟩
British Journal of Nutrition, Cambridge University Press (CUP), 2012, 108 (12), pp.2105-2114. ⟨10.1017/S000711451200027X ⟩
ISSN: 0007-1145
1475-2662
Popis: The first months of life correspond to a key period in human life where dramatic physiological changes (establishment of microbiota, development of the immune system, etc.) occur. In order to better control these changes it is necessary to understand the behaviour of food in the gastrointestinal tract of the newborn. Infant formula is the only food for the newborn when breast-feeding is impossible. The kinetics of digestion of milk proteins and the nature of the peptides liberated in the small intestine throughout infant formula digestion have never been extensively investigated so far and were therefore studied using the piglet as a model of the newborn child. Piglets were fed infant formula by an automatic delivery system during 28 d, and slaughtered 30, 90 and 210 min after the last meal. Contents of stomach, proximal and median jejunum and ileum were collected and characterised. The extent of β-lactoglobulin (β-lg), α-lactalbumin (α-la) and casein proteolysis was monitored by inhibition ELISA, SDS-PAGE, immunoblotting and MS. At 30 min after the last meal, caseins were shown to be extensively hydrolysed in the stomach. Nevertheless, peptides originating mainly from β-caseins (from 509 to 2510 Da) were identified in the jejunum and ileum of the piglets. β-Lg partially resisted gastric digestion but completely disappeared in the stomach after 210 min. α-La had a similar behaviour to that of β-lg. Two large peptides (4276 and 2674 Da) generated from β-lg were present in the ileum after 30 and 210 min and only one (2674 Da) after 90 min.
Databáze: OpenAIRE
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