Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis
| Autor: | Carolina Cunha Monteiro, Nágila Francinete Costa Secundino, Thais Bonifácio Campolina, Paulo Filemon Paolucci Pimenta, Luis Eduardo Martinez Villegas |
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| Rok vydání: | 2020 |
| Předmět: |
Bacterial Diseases
0301 basic medicine Serratia RC955-962 Bacillus Disease Vectors Pathology and Laboratory Medicine Medical Conditions 0302 clinical medicine Arctic medicine. Tropical medicine RNA Ribosomal 16S Medicine and Health Sciences Protozoans Leishmania Serratia infection biology Microbiota Eukaryota Bacterial Pathogens RNA Bacterial Infectious Diseases Medical Microbiology Public aspects of medicine RA1-1270 Pathogens Research Article 030231 tropical medicine Microbiology Serratia Infections Bacterial genetics 03 medical and health sciences Enterobacteriaceae Escherichia parasitic diseases Parasitic Diseases medicine Animals Microbial Pathogens Lysinibacillus Bacteria Gut Bacteria Organisms Public Health Environmental and Occupational Health Biology and Life Sciences Ribosomal RNA biology.organism_classification medicine.disease Parasitic Protozoans Insect Vectors Sand Flies Sandfly Species Interactions 030104 developmental biology Visceral leishmaniasis Psychodidae |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008666 (2020) |
| ISSN: | 1935-2735 |
| DOI: | 10.1371/journal.pntd.0008666 |
| Popis: | The microbial consortium associated with sandflies has gained relevance, with its composition shifting throughout distinct developmental stages, being strongly influenced by the surroundings and food sources. The bacterial components of the microbiota can interfere with Leishmania development inside the sandfly vector. Microbiota diversity and host-microbiota-pathogen interactions regarding New World sandfly species have yet to be thoroughly studied, particularly in Lutzomyia longipalpis, the primary vector of visceral leishmaniasis in Brazil.The native microbiota of different developmental stages and physiological conditions of Lu. longipalpis (Lapinha Cave), was described by culturing and 16s rRNA gene sequencing. The 16s rRNA sequencing of culture-dependent revealed 13 distinct bacterial genera (Bacillus, Enterococcus, Erwinia, Enterobacter, Escherichia, Klebsiella, Lysinibacillus, Pseudocitrobacter, Providencia, Pseudomonas, Serratia, Staphylococcus and Solibacillus). The in vitro and in vivo effects of each one of the 13 native bacteria from the Lu. longipalpis were analyzed by co-cultivation with promastigotes of L.i. chagasi, L. major, L. amazonensis, and L. braziliensis. After 24 h of co-cultivation, a growth reduction observed in all parasite species. When the parasites were co-cultivated with Lysinibacillus, all parasites of L. infantum chagasi and L. amazonensis died within 24 hours. In the in vivo co-infection of L.chagasi, L. major and L. amazonensis with the genera Lysinibacillus, Pseudocitrobacter and Serratia it was possible to observe a significant difference between the groups co-infected with the bacterial genera and the control group.These findings suggest that symbiont bacteria (Lysinibacillus, Serratia, and Pseudocitrobacter) are potential candidates for paratransgenic or biological control. Further studies are needed to identify the nature of the effector molecules involved in reducing the vector competence for Leishmania. Author summary According to the World Health Organization Leishmaniasis is the second parasitic disease that kills the most in the world; the first is malaria. Despite this, knowledge about the Leishmania parasite and its interaction with vertebrate hosts concerning the transmitting insect is still relatively fewer and fragmented. Studies on insects microbiota have great importance to obtain basic information. How a vector responds to the presence of different microorganisms and how they interact with various pathogens and may lead to the development of new strategies or tools that can be used to prevent or hinder the transmission of the protozoan by the vector insect. Considering the knowledge about the intestinal microbiota of sandflies, we aim to study the effect of bacterial isolates on Lu. longipalpis infection by different species of Leishmania, and it believed that these bacteria might influence the development of Leishmania, preventing, and hindering transmission, contributing to Leishmaniasis control strategies. |
| Databáze: | OpenAIRE |
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