Derivation of Man-1 and Man-2 research grade human embryonic stem cell lines
| Autor: | Rachel A. Oldershaw, Tristan R. McKay, Lisa Shaw, Nicola Bates, Daniel R. Brison, Maria V. Camarasa, Sharon Sneddon, Fiona Small, Melissa A. Baxter, Susan J. Kimber, Robbie W. Kerr |
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| Rok vydání: | 2010 |
| Předmět: |
Cellular differentiation
Cell Culture Techniques Fluorescent Antibody Technique Biology Cell Line Mice medicine Animals Humans Blastocyst Induced pluripotent stem cell Embryonic Stem Cells Cell Differentiation Embryo Cell Biology General Medicine Adaptation Physiological Embryonic stem cell Chromosome Banding Culture Media Cell biology Embryo Research medicine.anatomical_structure Cell culture Immunology Stem cell Developmental biology Biomarkers Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology - Animal. 46:386-394 |
| ISSN: | 1543-706X 1071-2690 |
| DOI: | 10.1007/s11626-010-9291-5 |
| Popis: | We report here the derivation of two new human embryonic stem cell lines, Man-1 and Man-2, and their full characterization as novel pluripotent stem cell lines. Man-1 was derived from an embryo surplus to requirement from routine IVF, while Man-2 was obtained from an oocyte classified as failed to fertilise and subsequently chemically activated. We report the characterisation of pluripotency and the differentiation potential of these lines. Work is in progress to establish novel methods of stem cell derivation and culture, which will avoid the use of xenobiotics and be relevant to clinical production of human embryonic stem cell lines. Both newly derived human embryonic stem cell lines will be available for the research community from the UK Stem Cell Bank (http://www.ukstemcellbank.org.uk). |
| Databáze: | OpenAIRE |
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