Tumor growth suppressionin vivoby overexpression of the circadian component, PER2
| Autor: | Norio Ishida, Yasuhiro Hara, Miyuki Wakabayashi, Koyomi Miyazaki |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Mice
Inbred ICR endocrine system biology Biochemical Phenomena Cell Cycle Cell Motility Growth Period Circadian Proteins Cell Biology Cell cycle Killer Cells Natural Fibronectin Mice Wee1 Cytolysis medicine.anatomical_structure Cell culture In vivo Neoplasms Genetics medicine biology.protein Cancer research Animals |
| Zdroj: | Genes to Cells. 15:351-358 |
| ISSN: | 1365-2443 1356-9597 |
| DOI: | 10.1111/j.1365-2443.2010.01384.x |
| Popis: | Some reports have indicated that the core clock gene, Per2 regulates the cell cycle, immune system and neural functions. To understand the effects of PER2 on tumor growth in vivo, stable transformants of murine sarcoma 180 (S-180) cell lines expressing different levels of PER2 were established. The growth of stable PER2 transformants in vivo was significantly and dose-dependently suppressed according to the amount of PER2 expressed, indicating that PER2 plays a role in the growth suppression of sarcoma cells. The anchorage-dependent and -independent growth in vitro and expression of the clock controlled cell-cycle related genes, wee1, myc, and VEGF were not altered in stable PER2 transformants. In contrast, susceptibility to murine natural killer (NK) cell cytolytic activity was enhanced in PER2 transformants. Furthermore, PER2 transformants suppressed cell motility and reduced fibronectin expression, but the expression of integrin receptors was not affected. These results suggest that sarcoma cells overexpressing PER2 suppress tumors in vivo by changing the nature of tumor cell adhesion. |
| Databáze: | OpenAIRE |
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