Lipoteichoic acid-related molecule derived from the streptococcal preparation, OK-432, which suppresses atopic dermatitis-like lesions in NC/Nga mice
| Autor: | SangJae Bae, Masato Okamoto, Ichiro Katayama, Yasuhiro Horiuchi, Mitsunobu Sato, Tetsuya Oshikawa |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Lipopolysaccharides
Ratón medicine.medical_treatment Anti-Inflammatory Agents Down-Regulation Suppressor of Cytokine Signaling Proteins Dermatology In situ hybridization Monocytes Dermatitis Atopic Picibanil Lesion Mice Th2 Cells Immune system Animals Medicine SOCS3 In Situ Hybridization Mice Inbred BALB C business.industry General Medicine Th1 Cells medicine.disease Immunohistochemistry Molecular biology Up-Regulation Teichoic Acids Cellular infiltration Cytokine Suppressor of Cytokine Signaling 3 Protein Models Animal Immunology Lipoteichoic acid medicine.symptom STAT6 Transcription Factor business |
| Zdroj: | Archives of Dermatological Research. 298:163-173 |
| ISSN: | 1432-069X 0340-3696 |
| DOI: | 10.1007/s00403-006-0674-0 |
| Popis: | Bacterial stimulation may serve to control atopic disorders such as atopic dermatitis (AD) through inducement of Th1 cell-mediated immune response. The lipoteichoic acid (LTA)-related molecule (okLTA) from streptococcal preparation, OK-432, has been shown to be a potent Th1 inducer through the action of IL-12. Examination was made of the therapeutic effects of this okLTA injected intra- and/or subcutaneously into AD-like lesions in NC/Nga mice, particularly in the vicinity of the suppressor of cytokine signaling (SOCS) regulatory pathways. Using immunohistochemical staining with IL-4/IL-12p40 and phosphorylated STAT6/p-STAT4 and RT-PCR for IL-4/IL-12p40, STAT6/STAT4 and mRNA expression and in situ hybridization of SOCS3 and 5, evaluation was made of the immunoregulatory effects of this okLTA in the treatment of spontaneous AD-like lesions in NC/Nga mice. Following the injection of okLTA, remarkable improvement in the lesions of NC/Nga mice was noted. In okLTA-treated skin, IL-12p40/p-STAT4 positive cellular infiltration was extensive while IL-4/p-STAT6 positive cell infiltration was seen to diminish considerably, compared to untreated NC mice. SOCS3 in situ expression in okLTA-treated mice was noted to be significantly less compared to untreated NC mice, in which the expression was prominent. SOCS5 in situ expression was rather, though not significantly, strong in okLTA-treated mice. okLTA treatment is clearly shown to induce Th1 cellular response and down-regulate immune response in the Th2 pathway through SOCS3 reduction in AD-like lesions of NC/Nga mice. The present results demonstrate that bacterial wall components such as okLTA should serve as an effective new therapeutic approach for treating AD. |
| Databáze: | OpenAIRE |
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