Mutations at the 3′ splice site can be suppressed by compensatory base changes in U1 snRNA in fission yeast
| Autor: | Claudia I. Reich, Gregory Porter, Roger W. Vanhoy, Jo Ann Wise |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Genetics
Base Composition Splice site mutation Base Sequence RNA Splicing Molecular Sequence Data Intron Prp24 Biology biology.organism_classification General Biochemistry Genetics and Molecular Biology Splicing factor Mutation Schizosaccharomyces RNA splicing Schizosaccharomyces pombe snRNP RNA Processing Post-Transcriptional Small nuclear RNA |
| Zdroj: | Cell. 69:1159-1169 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/0092-8674(92)90637-r |
| Popis: | U1 snRNA is an essential splicing factor known to base pair with 5' splice sites of premessenger RNAs. We demonstrate that pairing between the universally conserved CU just downstream from the 5' junction interaction region and the 3' splice site AG contributes to efficient splicing of Schizosaccharomyces pombe introns that typify the AG-dependent class described in mammals. Strains carrying mutations in the 3' AG of an artificial intron accumulate linear precursor, indicative of a first step block. Lariat formation is partially restored in these mutants by compensatory changes in nucleotides C7 and U8 of U1 snRNA. Consistent with a general role in fission yeast splicing, mutations at C7 are lethal, while U8 mutants are growth impaired and accumulate linear, unspliced precursor to U6 snRNA. U1 RNA-mediated recognition of the 3' splice site may have origins in analogous intramolecular interactions in an ancestral self-splicing RNA. |
| Databáze: | OpenAIRE |
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