U6 snRNA intron insertion occurred multiple times during fungi evolution
| Autor: | Jana Hertel, Sebastian Canzler, Peter F. Stadler |
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| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
intron RNA Family RNA Splicing Prp24 snRNA evolution Evolution Molecular 03 medical and health sciences snRNA RNA Small Nuclear Sequence Homology Nucleic Acid Schizosaccharomyces snRNP homology search Molecular Biology Gene Genetics biology Base Sequence Intron Cell Biology Group II intron biology.organism_classification Introns 030104 developmental biology RNA splicing Nucleic Acid Conformation fungi Genome Fungal Small nuclear RNA |
| Popis: | U6 small nuclear RNAs are part of the splicing machinery. They exhibit several unique features setting them appart from other snRNAs. Reports of introns in structured non-coding RNAs have been very rare. U6 genes, however, were found to be interrupted by an intron in several Schizosaccharomyces species and in 2 Basidiomycota. We conducted a homology search across 147 currently available fungal genome and identified the U6 genes in all but 2 of them. A detailed comparison of their sequences and predicted secondary structures showed that intron insertion events in the U6 snRNA were much more common in the fungal lineage than previously thought. Their positional distribution across the entire mature snRNA strongly suggests a large number of independent events. All the intron sequences reported here show canonical splice site and branch site motifs indicating that they require the splicesomal pathway for their removal. |
| Databáze: | OpenAIRE |
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