Immunohistochemistry for identification of CCND1, NSD2, and MAF gene rearrangements in plasma cell myeloma
| Autor: | Hiroshi Inagaki, Shinsuke Iida, Takayuki Murase, Tomoko Narita, Ayako Masaki, Keiichiro Fujii, Masaki Ri |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Cancer Research gene rearrangements Biology Sensitivity and Specificity FFPE tissue sections Cohort Studies 03 medical and health sciences 0302 clinical medicine Cyclin D1 Clinical Research hemic and lymphatic diseases Plasma Cell Myeloma medicine Humans Gene Multiple myeloma Aged Aged 80 and over Gene Rearrangement medicine.diagnostic_test Fish analysis General Medicine Histone-Lysine N-Methyltransferase Original Articles Middle Aged medicine.disease Molecular biology Immunohistochemistry Repressor Proteins multiple myeloma 030104 developmental biology tissue fluorescence in situ hybridization Oncology 030220 oncology & carcinogenesis Proto-Oncogene Proteins c-maf Risk stratification Female Original Article Fluorescence in situ hybridization |
| Zdroj: | Cancer Science |
| ISSN: | 1349-7006 |
| Popis: | The t(11;14)/CCND1‐IGH, t(4;14)/NSD2(MMSET)‐IGH, and t(14;16)/IGH‐MAF gene rearrangements detected by fluorescence in situ hybridization (FISH) are used for risk stratification in patients with multiple myeloma (MM). Compared with conventional FISH techniques using fresh cells, immunohistochemistry (IHC) is much more cost‐ and time‐efficient, and can be readily applied to routinely prepared formalin‐fixed, paraffin‐embedded (FFPE) materials. In this study, we performed tissue FISH and IHC employing FFPE specimens, and examined the usefulness of IHC as a tool for detecting CCND1,NSD2, and MAF gene rearrangements. CD138 signals were used to identify plasma cells in tissue FISH and IHC analyses. With cohort 1 (n = 70), we performed tissue FISH and subsequently IHC, and determined IHC cut‐off points. In this cohort, the sensitivity and specificity for the 3 molecules were ≥.90 and ≥.96, respectively. With cohort 2, using MM cases with an unknown gene status (n = 120), we performed IHC, and the gene status was estimated using the cut‐off points determined with cohort 1. The subsequent FISH analysis showed that the sensitivity and specificity for the 3 molecules were ≥.92 and ≥.98, respectively. CCND1, NSD2, and MAF gene rearrangements were estimated accurately by IHC, suggesting that conventional FISH assays can be replaced by IHC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text