Roflumilast as a Potential Therapeutic Agent for Cecal Ligation and Puncture-Induced Septic Lung Injury
| Autor: | Arzu Bilen, Zafer Bayraktutan, Erdem Toktay, Zekai Halici, Busra Sirin, Duygu Kose, Halil Keskin, Busra Dincer |
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| Rok vydání: | 2021 |
| Předmět: |
Cyclopropanes
animal diseases Aminopyridines Inflammation Punctures Pharmacology Lung injury medicine.disease_cause digestive system Sepsis 03 medical and health sciences 0302 clinical medicine Phosphodiesterase-4 medicine Animals Cecum Ligation Lung Roflumilast business.industry NF-kappa B Cecal ligation Lung Injury bacterial infections and mycoses medicine.disease Rats Disease Models Animal 030220 oncology & carcinogenesis Benzamides 030211 gastroenterology & hepatology Surgery medicine.symptom business Oxidative stress medicine.drug |
| Zdroj: | Journal of Investigative Surgery. 35:605-613 |
| ISSN: | 1521-0553 0894-1939 |
| Popis: | This study focused on delineating the possible effects of roflumilast (ROF), a selective phosphodiesterase 4 (PDE4) inhibitor, in rats with cecal ligation and puncture (CLP)-induced polymicrobial sepsis, and investigated whether ROF can act as a protective agent in sepsis-induced lung damage.Four experimental groups were organized, each comprising eight rats: Control, Sepsis, Sepsis + ROF 0.5 mgkgIn the sepsis group's lungs, the TNF-α, IL-1β, and IL-6 mRNA expression levels peaked in the sepsis group's lung tissues, and ROF significantly decreased these levels compared with the sepsis group dose-dependently. ROF also significantly decreased MDA levels in septic lungs and increased antioxidant parameters (SOD and GSH) compared with the sepsis group. Histopathological analysis results supported biochemical and molecular results.ROF, a PDE4 inhibitor, suppressed the expression levels of pro-inflammatory cytokines, alleviated lung damage (probably by blocking neutrophil infiltration), and increased the capacity of the antioxidant system. |
| Databáze: | OpenAIRE |
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