Exploring the extremes: applying high concentration of yeast extract leads to drastic morphological changes and elimination of (+)-geodin and asterric acid production in Aspergillus terreus submerged cultures
| Autor: | Marcin Bizukojc, Ida Stefaniak, Adrianna Górnicka, Iwona Grzybowska, Tomasz Boruta |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Hypha
Bioengineering Saccharomyces cerevisiae Applied Microbiology and Biotechnology chemistry.chemical_compound Fungal morphology medicine Yeast extract Aspergillus terreus Food science Lovastatin Lactose Sugar Mycelium Benzofurans Biological Products biology Chemistry Secondary metabolites Phenyl Ethers Substrate (chemistry) General Medicine biology.organism_classification Original Research Paper Aspergillus Biotechnology medicine.drug |
| Zdroj: | Biotechnology Letters |
| ISSN: | 1573-6776 0141-5492 |
| Popis: | Objective Evaluation of morphology and secondary metabolites production in Aspergillus terreus ATCC 20542 cultures over a wide range of lactose and yeast extract concentrations from 0.2 up to an extremely high level of 200 g l−l. Results The morphological differences of mycelial objects were quantified with the use of morphological parameters calculated by applying the tools of digital image analysis. At 200 g l−l of yeast extract clumps and loose hyphae were recorded instead of pellets commonly observed in submerged cultures of A. terreus. Under these conditions the biosynthesis of (+)-geodin and asterric acid was totally blocked, lovastatin formation was found to be at a relatively low level and biomass production turned out to be greater than in the remaining variants, where the pelleted growth was observed. At 200 g l−l of lactose the production of lovastatin, (+)-geodin and asterric acid was visibly stimulated compared to the media containing 0.2, 2 and 20 g l−l of the sugar substrate, but at the same time no traces of butyrolactone I could be detected in the broth. Lactose at the extremely high concentration of 200 g l−l did not induce the drastic morphological changes observed in the case of 200 g l-1 of yeast extract. It was proved that at the C/N values as low as 4 and as high as 374 A. terreus not only continued to display growth but also exhibited the production of secondary metabolites. The use of cultivation media representing the equivalent C/N ratios led to different metabolic and morphological outcomes depending on the concentration of lactose and yeast extract that contributed to the given C/N value. Conclusion The extremely high concentration of yeast extract leads to marked morphological changes of A. terreus and the elimination of (+)-geodin and asterric production, while applying the excess of lactose is stimulatory in terms of lovastatin production. |
| Databáze: | OpenAIRE |
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