Heat Shock Induces Interferon-TAU Gene Expression byIn Vitro-Produced Bovine Blastocysts
| Autor: | Alison Ainslie, Cheryl Ashworth, John Rooke, Cristina F. Hickman, Michael Clinton |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Immunology
Pregnancy Proteins Biology Andrology Pregnancy Interferon Gene expression medicine Animals Immunology and Allergy HSP70 Heat-Shock Proteins Blastocyst Messenger RNA Gene Expression Regulation Developmental Obstetrics and Gynecology Embryo Molecular biology Interferon tau Hsp70 medicine.anatomical_structure Reproductive Medicine Shock (circulatory) Interferon Type I embryonic structures Cattle Female medicine.symptom Heat-Shock Response medicine.drug |
| Zdroj: | American Journal of Reproductive Immunology. 70:177-181 |
| ISSN: | 1046-7408 |
| DOI: | 10.1111/aji.12131 |
| Popis: | Problem The type I interferon (IFN), IFN-tau (τ), is the primary embryonic signal for pregnancy maintenance in ruminants. This study determined the effects of heat shock upon IFN-τ (IFNT) gene expression by bovine blastocysts in vitro. Method of study In vitro-produced blastocyst-stage embryos were exposed to 42°C for 4 hr, and mRNA for heat-shock protein 70 (HSP70) and IFNT quantified. Results Heat shock increased both HSP70 and IFNT expression. There was a significant correlation between HSP70 and IFNT transcript levels irrespective of whether a blastocyst had been exposed to heat shock or not. Conclusion The increase in IFNT as a result of heat shock suggests that a proportion of the variation in IFNT expression observed in blastocyst-stage embryos is a response to stress. |
| Databáze: | OpenAIRE |
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