Homologous prime-boost with Zika virus envelope protein and poly (I:C) induces robust specific humoral and cellular immune responses
Autor: | Silvia Beatriz Boscardin, Marcelo Pires Amaral, Nádia Tomita, Renato Mancini Astray, Victória Alves Santos Lunardelli, Fernanda Caroline Coirada, Juliana de Souza Apostólico, Higo Fernando Santos Souza, Edgar Ruz Fernandes, Daniela Santoro Rosa |
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Rok vydání: | 2019 |
Předmět: |
CpG Oligodeoxynucleotide
medicine.medical_treatment 030231 tropical medicine Immunization Secondary Heterologous Biology Virus DNA vaccination law.invention 03 medical and health sciences Mice 0302 clinical medicine Immune system Viral Envelope Proteins law medicine Vaccines DNA Animals 030212 general & internal medicine DNA RECOMBINANTE Immunity Cellular Mice Inbred BALB C General Veterinary General Immunology and Microbiology Zika Virus Infection Public Health Environmental and Occupational Health Zika Virus Virology Infectious Diseases Immunization Viral Envelope Recombinant DNA Molecular Medicine Adjuvant |
Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
ISSN: | 1873-2518 |
Popis: | The recent outbreaks of Zika virus (ZIKV) infection and the potential association with Guillain-Barre syndrome in adults and with congenital abnormalities have highlighted the urgency for an effective vaccine. The ZIKV Envelope glycoprotein (EZIKV) is the most abundant protein on the virus surface, and has been evaluated together with the pre-membrane protein (prM) of the viral coat as a vaccine candidate in clinical trials. In this study, we performed a head-to-head comparison of the immune response induced by different EZIKV-based vaccine candidates in mice. We compared different platforms (DNA, recombinant protein), adjuvants (poly (I:C), CpG ODN 1826) and immunization strategies (homologous, heterologous). The hierarchy of adjuvant potency showed that poly (I:C) was a superior adjuvant than CpG ODN. While poly (I:C) assisted immunization reached a plateau in antibody titers after two doses, the CpG ODN group required an extra immunization dose. Besides, the administration of poly (I:C) induced higher EZIKV-specific cellular immune responses than CpG ODN. We also show that immunization with homologous prime-boost EZIKV protein + poly (I:C) regimen induced a more robust humoral response than homologous DNA (pVAX-EZIKV) or heterologous regimens (DNA/protein or protein/DNA). A detailed analysis of cellular immune responses revealed that homologous (EZIKV + poly (I:C)) and heterologous (pVAX-EZIKV/EZIKV + poly (I:C)) prime-boost regimens induced the highest magnitude of IFN-γ secreting cells and cytokine-producing CD4+ T cells. Overall, our data demonstrate that homologous EZIKV + poly (I:C) prime-boost immunization is sufficient to induce more robust specific-EZIKV humoral and cellular immune responses than the other strategies that contemplate homologous DNA (pVAX-EZIKV) or heterologous (pVAX-EZIKV/EZIKV + poly (I:C), and vice-versa) immunizations. |
Databáze: | OpenAIRE |
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