Abundance, community structure and diversity of nitrifying bacterial enrichments from low and high saline brackishwater environments
| Autor: | Satheesha Avunje, M. S. Shekhar, M. Leo-Antony, Shankar Vinayakarao Alavandi, T.N. Vinay, Prasanna Kumar Patil, Koyadan Kizhakedath Vijayan, Viswanathan Baskaran |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Salinity Nitrosopumilus mothur 01 natural sciences Applied Microbiology and Biotechnology Nitrospirae 03 medical and health sciences Chromatiaceae Nitrososphaera RNA Ribosomal 16S 010608 biotechnology Botany Saline Waters Nitrosomonas Population Density 0303 health sciences Bacteria biology 030306 microbiology Microbiota Biodiversity biology.organism_classification Archaea Nitrification Ectothiorhodospiraceae Nitrospira |
| Zdroj: | Letters in Applied Microbiology. 73:96-106 |
| ISSN: | 1472-765X 0266-8254 |
| DOI: | 10.1111/lam.13480 |
| Popis: | The study reports diversity in nitrifying microbial enrichments from low (0·5-5‰) and high (18-35‰) saline ecosystems. Microbial community profiling of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) enrichments was analysed by sequencing 16S rRNA and was processed using Mothur pipeline. The α-diversity indices showed the richness of nitrifying bacterial consortia from the high saline environment and were clustering based on the source of the sample. AOB and NOB enrichments from both the environments showed diverse lineages of phyla distributed in both groups with 38 and 34 phyla from low saline and 53 and 40 phyla in high saline sources, respectively. At class level, α- and γ-proteobacteria were found to be more dominant in both the enrichments. AOBs and NOBs in enrichments from low saline environments were dominated by Nitrosomonadaceae, Gallionellaceae (Nitrotoga sp.) and Ectothiorhodospiraceae and Nitrospira, respectively. Though Chromatiaceae were present in both AOB and NOB enrichments, Nitrosoglobus and Nitrosococcus dominated the AOBs while NOBs were dominated by uncultured genera, whereas Rhizobiales were found in both the enrichments. AOBs and NOBs in enrichments from high saline environments were dominated by Nitrospira-like AOBs, Nitrosomonas and Nitrosococcus genera, whereas ammonia-oxidizing archaea (AOA) group included Nitrosopumilus and Nitrososphaera genera comprising and Nitrospirae, respectively. The majority of the genera obtained in both the salinities were found to be either uncultured or unclassified groups. Results of the study suggest that the AOB and NOB consortia have unique and diverse microbes in each of the enrichments, capable of functioning in aquaculture systems practised at different salinities (0-60 ppt). |
| Databáze: | OpenAIRE |
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