The tapeworm Ligula intestinalis (Cestoda: Pseudophyllidea) inhibits LH expression and puberty in its teleost host, Rutilus rutilus
| Autor: | S. Dufour, C Arme, Raymond J. Pierce, D Hoole, V. Carter |
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| Přispěvatelé: | Biologie des organismes marins et écosystèmes (BOME), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Muséum national d'Histoire naturelle (MNHN) |
| Rok vydání: | 2005 |
| Předmět: |
Embryology
Pituitary gland medicine.medical_specialty medicine.drug_class Cestoda Cyprinidae Host-Parasite Interactions 030308 mycology & parasitology 03 medical and health sciences Endocrinology Internal medicine medicine Animals RNA Messenger Sexual Maturation Ligula intestinalis Carp [SDV.BDD]Life Sciences [q-bio]/Development Biology 030304 developmental biology Primary oocyte stage 0303 health sciences biology Reverse Transcriptase Polymerase Chain Reaction Obstetrics and Gynecology Cell Biology Luteinizing Hormone biology.organism_classification medicine.anatomical_structure Reproductive Medicine Pituitary Gland Oocytes Body Burden Female Seasons Rutilus Development of the gonads Gonadotropin |
| Zdroj: | Reproduction (Cambridge, England) Reproduction (Cambridge, England), 2005, 130, pp.939-945 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep.1.00742 |
| Popis: | The tapewormLigula intestinalisoccurs in the body cavity of its cyprinid second intermediate host, in this study the roachRutilus rutilus, and inhibits host gonadal development. The mechanism by which infected fish are prevented from reproducing is unknown. Comparison of parameters, such as body length and weight, and condition factor and age, between infected and uninfected individuals, indicated only minor effects of parasitism on growth and condition. In contrast, seasonal gonadal development, as observed in uninfected fish, did not occur in infected fish, and gonads remained small and blocked at the primary oocyte stage in female roach. As immature ovaries and testes are still present, the parasite is presumed to act upon the brain–pituitary–gonadal axis of the fish to inhibit further development of reproductive organs. We investigated theLigula/fish interaction at the level of the pituitary gland by determination of gonadotrophin (LH) content using a heterologous RIA for carp (Cyprinus carpio) LHβ subunit. The results indicated that the pituitary glands of infected roach contained approximately 50% less LH than non-infected fish. After the cloning and sequencing of roach LHβ subunit, we measured roach LHβ mRNA levels by real-time RT-PCR. A corresponding 50% reduction in LHβ mRNA pituitary levels was determined. These results reflect a significant and measurable effect of parasitism on the pituitary gland, and lend support to the hypothesis that excretory/secretory products released from the parasite interact with the brain–pituitary–gonadal axis of the fish host and thus inhibit gonadal development. |
| Databáze: | OpenAIRE |
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