Microbial HemG-type protoporphyrinogen IX oxidase enzymes for biotechnology applications in plant herbicide tolerance traits
| Autor: | James K. Roberts, Oscar C Sparks, Arlene Howe, Artem G Evdokimov, Daniela N. Ribeiro, Zhou Xuefeng, Christine Ellis, Qi Qungang, Marguerite J Varagona, Janice R. Weihe, Xiaoping Wei, Steven T Voss, Erin Hall, Goley Michael E, Larue Clayton T, Shirley X. Guo, Joel E Ream, Farhard Moshiri |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
food.ingredient Biology 01 natural sciences Zea mays chemistry.chemical_compound food Protoporphyrinogen Oxidase Enzyme family Canola Heme chemistry.chemical_classification business.industry Herbicides food and beverages Protoporphyrinogen IX Oxidase General Medicine Biotechnology 010602 entomology Sequence homology Enzyme chemistry Insect Science Chlorophyll Plant species Soybeans business Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Pest management scienceREFERENCES. 76(3) |
| ISSN: | 1526-4998 |
| Popis: | Background Protoporphyrinogen IX oxidase (PPO)-inhibiting herbicides act by inhibiting a key enzyme in the heme and chlorophyll biosynthetic pathways in plants. This enzyme, the PPO enzyme, is conserved across plant species. However, some microbes are known to utilize a unique family of PPO enzymes, the HemG family. This enzyme family carries out the same enzymatic step as the plant PPO enzymes, but does not share sequence homology with the plant PPO enzymes. Results Bioinformatic analysis was used to identify putative HemG PPO enzyme variants from microbial sources. A subset of these variants was cloned and characterized. HemG PPO variants were characterized for functionality and tolerance to PPO-inhibiting herbicides. HemG PPO variants that exhibited insensitivity to PPO-inhibiting herbicides were identified for further characterization. Expression of selected variants in maize, soybean, cotton and canola resulted in plants that displayed tolerance to applications of PPO-inhibiting herbicides. Conclusion Selected microbial-sourced HemG PPO enzyme variants present an opportunity for building new herbicide tolerance biotechnology traits. These traits provide tolerance to PPO-inhibiting herbicides and, therefore, could provide additional tools for farmers to employ in their weed management systems. © 2019 Society of Chemical Industry. |
| Databáze: | OpenAIRE |
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