Diazepam-insensitive GABAA receptors on postnatal spiral ganglion neurones in culture
| Autor: | Thomas R. Van De Water, Frédéric Goffin, Paul Coucke, Shibeshih Belachew, Gustave Moonen, Philippe Lefèbvre, Jean-Michel Rigo, Brigitte Malgrange, Gaël Xhauflaire |
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| Rok vydání: | 1997 |
| Předmět: |
Agonist
Baclofen Patch-Clamp Techniques medicine.drug_class Convulsants Flunitrazepam Biology Bicuculline gamma-Aminobutyric acid Membrane Potentials chemistry.chemical_compound medicine Animals Picrotoxin Channel blocker Rats Wistar Pentobarbital Spiral ganglion Cells Cultured gamma-Aminobutyric Acid Neurons Diazepam GABAA receptor General Neuroscience Receptors GABA-A Rats Zinc medicine.anatomical_structure nervous system chemistry Animals Newborn Competitive antagonist Biophysics sense organs Spiral Ganglion Neuroscience medicine.drug Carbolines |
| Zdroj: | Neuroreport. 8(3) |
| ISSN: | 0959-4965 |
| Popis: | Using dissociated spiral ganglion cell cultures obtained from 3-day-old rat cochlea, we investigated the response of auditory neurones to gamma-aminobutyric acid (GABA) using patch-clamp techniques. In our recording conditions, GABA elicited inward currents in > 95% of the neurones which reversed around 0 mV. Similar inward currents were measured using isoguvacin, a specific agonist of GABAA receptors. GABA-gated currents were reversibly inhibited by the channel blocker picrotoxin and the GABA competitive antagonist bicuculline. These functional GABAA receptors are characterized by an insensitivity to benzodiazepines and a relatively high sensitivity to beta-carbolines and barbiturates. These results show that the GABAA receptor pharmacological properties of spiral ganglion neurones are close to those of cerebellar granule cells. |
| Databáze: | OpenAIRE |
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