Genome-wide comprehensive analysis of transcriptomes and small RNAs offers insights into the molecular mechanism of alkaline stress tolerance in a citrus rootstock
| Autor: | Hualin Yi, Junying Cao, Yanhui Xu, Juxun Wu, Mei Su, Guizhi Feng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Science Horticulture Biology 01 natural sciences Biochemistry Article Transcriptome 03 medical and health sciences food Auxin lcsh:Botany Genetics Jasmonate Citrus rootstock lcsh:QH301-705.5 chemistry.chemical_classification Auxin homeostasis food and beverages Citrus junos biology.organism_classification food.food Trifoliate orange lcsh:QK1-989 030104 developmental biology chemistry lcsh:Biology (General) Rootstock 010606 plant biology & botany Biotechnology |
| Zdroj: | Horticulture Research, Vol 6, Iss 1, Pp 1-19 (2019) Horticulture Research |
| ISSN: | 2052-7276 |
| Popis: | Alkaline stress has serious-negative effects on citrus production. Ziyang xiangcheng (Citrus junos Sieb. ex Tanaka) (Cj) is a rootstock that is tolerant to alkaline stress and iron deficiency. Trifoliate orange (Poncirus trifoliata (L.) Raf.) (Pt), the most widely used rootstock in China, is sensitive to alkaline stress. To investigate the molecular mechanism underlying the tolerance of Cj to alkaline stress, next-generation sequencing was employed to profile the root transcriptomes and small RNAs of Cj and Pt seedlings that were cultured in nutrient solutions along a three pH gradient. This two-level regulation data set provides a system-level view of molecular events with a precise resolution. The data suggest that the auxin pathway may play a central role in the inhibitory effect of alkaline stress on root growth and that the regulation of auxin homeostasis under alkaline stress is important for the adaptation of citrus to alkaline stress. Moreover, the jasmonate (JA) pathway exhibits the opposite response to alkaline stress in Cj and Pt and may contribute to the differences in the alkaline stress tolerance and iron acquisition between Cj and Pt. The dataset provides a wealth of genomic resources and new clues to further study the mechanisms underlying alkaline stress resistance in Cj. Molecular biology: Rooting out alkaline resistance mechanisms in citrus trees The ability of citrus trees to tolerate alkaline soils may hinge upon plant hormones called auxins which regulate root growth. The discovery could aid the development of more resilient rootstocks. Alkaline stress, caused by e.g. industrial run-off, is a growing problem worldwide because it reduces the growth and survival of crops – including the most widely used citrus rootstock in China, Poncirus trifoliata. To better understand the mechanisms underpinning tolerance to soil alkalinity, Hualin Yi at Huazhong Agricultural University in Wuhan, China, and colleagues used next-generation sequencing to profile the transcription products of P. trifolata seedlings and those of an alkaline-tolerant rootstock, Ziyang xiangcheng, when they were grown in three different nutrient solutions. Auxin homeostasis appears to be a key element of citrus adaption to alkaline stress, they found - probably by encouraging lateral root branching. |
| Databáze: | OpenAIRE |
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