HIF-3α affects preeclampsia development by regulating EVT growth via activation of the Flt-1/JAK/STAT signaling pathway in hypoxia
| Autor: | Hongmei Qu, Bei Jia, Qun Yu, Wenzhe Zhou, Linsong Mu, Yinghong Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult STAT3 Transcription Factor Cancer Research Cell Survival Fms-like tyrosine kinase receptor 1 Placenta Down-Regulation Apoptosis Biochemistry stat Cell Line preeclampsia 03 medical and health sciences 0302 clinical medicine Pre-Eclampsia Cell Movement Pregnancy Genetics Humans Viability assay Hypoxia Molecular Biology Cell Proliferation hypoxia-inducible factor-3α Vascular Endothelial Growth Factor Receptor-1 extravillous cytotrophoblast Chemistry JAK-STAT signaling pathway Articles Cell cycle Janus Kinase 2 Trophoblasts Up-Regulation Repressor Proteins 030104 developmental biology Oncology 030220 oncology & carcinogenesis embryonic structures Janus kinase-signal transducer 2/signal transducer and activator of transcription 3 signaling pathway STAT protein Cancer research Molecular Medicine Phosphorylation Female Janus kinase Apoptosis Regulatory Proteins Signal Transduction |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Preeclampsia (PE) is a common obstetric disease occurring after 20 weeks of gestation. Hypoxia‑inducible factor (HIF)‑3α potentially functions as a regulatory factor in PE development, however its specific molecular mechanism remains to be elucidated. The present study aimed to investigate the function of HIF‑3α in trophoblast cell line HTR‑8/SVneo, to provide a better understanding of the pathology and treatment of PE. Normal and PE placentas were obtained from pregnant women. HTR8/SVneo cells were cultured under the condition of normoxia or hypoxia, pretreated with or without AG490, then transfected with HIF‑3α. The gene expression levels of HIF‑3α and Fms like tyrosine kinase receptor (Flt) 1 extracted from the placentas and cells were detected by reverse transcription‑quantitative PCR, and the expression levels of proteins and Janus kinase signal transducer and activator of transcription (JAK/STAT) phosphorylation were detected by western blot analysis. Viability and apoptosis of the treated cells were assessed by MTT and flow cytometry. The results demonstrated that HIF‑3α and Flt‑1 gene expression levels of PE placentas were reduced compared with normal placentas. Under a hypoxic environment, the expression levels of HIF‑3α and Flt‑1, the phosphorylation of JAK/STAT and the cell viability of HTR8/SVneo cells were increased at first and then reduced, whereas cell apoptosis was promoted over time. Under chronic hypoxia, the expression levels of HIF‑3α and Flt‑1, JAK/STAT pathway phosphorylation and cell viability of AG490‑treated HTR8/SVneo cells were reduced, but cell apoptosis was promoted. However, the upregulation of HIF‑3α in HTR8/SVneo cells markedly reversed the effects of AG490 on the cells under hypoxia. Thus, the present study preliminarily demonstrated that HIF‑3α was involved in PE development by regulating extravillous cytotrophoblast growth via Flt‑1 and the JAK/STAT signaling pathway. |
| Databáze: | OpenAIRE |
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