The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms
| Autor: | Emanuela Di Campli, Soraya Di Bartolomeo, Eleonora Marsich, A. Cataldi, Mara Di Giulio, Silvia Sancilio, Andrea Travan, Luigina Cellini |
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| Přispěvatelé: | Di Giulio, M, Di Bartolomeo, S, Di Campli, E, Sancilio, S, Marsich, Eleonora, Travan, Andrea, Cataldi, A, Cellini, L. |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Silver silver-nanoparticle Metal Nanoparticles Biology Bacterial growth medicine.disease_cause urologic and male genital diseases Article Catalysis Silver nanoparticle Microbiology Inorganic Chemistry lcsh:Chemistry silver-nanoparticles saliva antimicrobial activity oral Streptococci planktonic and sessile growth Anti-Infective Agents Streptococcus mitis medicine Humans polysaccharide Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy Streptococcus urogenital system Organic Chemistry Biofilm General Medicine Antimicrobial biology.organism_classification Streptococcus mutans Computer Science Applications Streptococcus oralis lcsh:Biology (General) lcsh:QD1-999 Biofilms Female |
| Zdroj: | International Journal of Molecular Sciences, Vol 14, Iss 7, Pp 13615-13625 (2013) International Journal of Molecular Sciences; Volume 14; Issue 7; Pages: 13615-13625 International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Popis: | In this work, we studied the antimicrobial properties of a nanocomposite system based on a lactose-substituted chitosan and silver nanoparticles: Chitlac-nAg. Twofold serial dilutions of the colloidal Chitlac-nAg solution were both tested on Streptococcus mitis, Streptococcus mutans, and Streptococcus oralis planktonic phase and biofilm growth mode as well as on saliva samples. The minimum inhibitory and bactericidal concentrations of Chitlac-nAg were evaluated together with its effect on sessile cell viability, as well as both on biofilm formation and on preformed biofilm. In respect to the planktonic bacteria, Chitlac-nAg showed an inhibitory/bactericidal effect against all streptococcal strains at 0.1% (v/v), except for S. mitis ATCC 6249 that was inhibited at one step less. On preformed biofilm, Chitlac-nAg at a value of 0.2%, was able to inhibit the bacterial growth on the supernatant phase as well as on the mature biofilm. For S. mitis ATCC 6249, the biofilm inhibitory concentration of Chitlac-nAg was 0.1%. At sub-inhibitory concentrations, the Streptococcal strains adhesion capability on a polystyrene surface showed a general reduction following a concentration-dependent-way; a similar effect was obtained for the metabolic biofilm activity. From these results, Chitlac-nAg seems to be a promising antibacterial and antibiofilm agent able to hinder plaque formation. |
| Databáze: | OpenAIRE |
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