T2 and Apparent Diffusion Coefficient of MRI Reflect Maturation of Tissue-Engineered Auricular Cartilage Subcutaneously Transplanted in Rats
| Autor: | Naotaka Nitta, Yuko Fujihara, Kazuhiko Hayashi, Tomokazu Numano, Kazuhiro Homma, Kuni Ohtomo, Ryo Kosaka, Koji Hyodo, Yoshio Shirasaki, Shouta Kuribayashi, Yasushi Watanabe, Tsuyoshi Takato, Kazuto Hoshi, Masaki Misawa, Jiro Sato |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Biomedical Engineering Type II collagen Medicine (miscellaneous) Bioengineering 01 natural sciences Regenerative medicine 030218 nuclear medicine & medical imaging Rats Nude 03 medical and health sciences 0302 clinical medicine 0103 physical sciences otorhinolaryngologic diseases medicine Animals Humans Effective diffusion coefficient Autologous chondrocyte implantation Cells Cultured 010302 applied physics Tissue Engineering Tissue Scaffolds medicine.diagnostic_test business.industry Cartilage Ultrasound Cell Differentiation Magnetic resonance imaging Magnetic Resonance Imaging Rats Transplantation medicine.anatomical_structure Ear Cartilage business Biomedical engineering |
| Zdroj: | Tissue Engineering Part C: Methods. 22:429-438 |
| ISSN: | 1937-3392 1937-3384 |
| Popis: | In cartilage regenerative medicine, autologous chondrocyte implantation (ACI) has been applied clinically for partial defects of joint cartilage or nasal augmentation. To make treatment with ACI more effective and prevalent, modalities to evaluate the quality of transplanted constructs noninvasively are necessary. In this study, we compared the efficacy of several noninvasive modalities for evaluating the maturation of tissue-engineered auricular cartilage containing a biodegradable polymer scaffold. We first transplanted tissue-engineered cartilage consisting of human auricular chondrocytes, atelocollagen gel, and a poly-l-lactic acid (PLLA) porous scaffold subcutaneously into the back of athymic nude rats. Eight weeks after transplantation, the rats were examined by magnetic resonance imaging (MRI), X-ray, and ultrasound as noninvasive modalities. Then, the excised constructs were examined by histological and biochemical analysis including toluidine blue (TB) staining, glycosaminoglycans content, and enzyme-linked immunosorbent assay of type II collagen. Among the modalities examined, transverse relaxation time (T2) and apparent diffusion coefficient of MRI showed quite a high correlation with histological and biochemical results, suggesting that these can effectively detect the maturation of tissue-engineered auricular cartilage. Since these noninvasive modalities would realize time-course analysis of the maturation of tissue-engineered auricular cartilage, this study provides a substantial insight for improving the quality of tissue-engineered cartilage, leading to improvement of the quality and technique in cartilage regenerative medicine. |
| Databáze: | OpenAIRE |
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