Human follicular fluid from superovulated women inhibits progesterone receptor-dependent gonadotropin-releasing hormone self-priming in an estrous cycle-dependent manner in the rat
| Autor: | Yolanda Millán, Carmina Bellido, José C. Garrido-Gracia, Silvia Guil-Luna, J. Martín de las Mulas, José E. Sánchez-Criado, Ana Gordon, Rafaela Aguilar, E. Bellido-Muñoz, Juan A. Garcia-Velasco |
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| Rok vydání: | 2010 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class Endocrinology Diabetes and Metabolism Estrous Cycle Superovulation Gonadotropin-releasing hormone Gonadotropic cell Gonadotropin-Releasing Hormone Endocrinology Internal medicine Follicular phase Progesterone receptor medicine Animals Humans Rats Wistar Estrous cycle Chemistry Proteins Luteinizing Hormone Follicular fluid Follicular Fluid Rats Gonadotropin secretion Pituitary Gland Female Follicle Stimulating Hormone Gonadotropin Receptors Progesterone Gonadal Hormones hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Endocrinological Investigation. 33:564-570 |
| ISSN: | 1720-8386 0391-4097 |
| DOI: | 10.1007/bf03346649 |
| Popis: | These experiments investigated the involvement of gonadotrope progesterone receptor (PR) in the effects of the putative gonadotropin surge-attenuating factor (GnSAF) on gonadotropin (LH and FSH) secretion. Human follicular fluids (hFF) used in this study were aspirated from follicles in gonadotropin-treated women for in vitro fertilization. Samples were subjected to two-fold charcoal extraction of steroid hormones and two-fold inhibin immunoprecipitation. Gonadotropin secretion parameters were assessed by specific radioimmunoassays. In the first experiment, the effects of hFF on both basal and GnRH-stimulated gonadotropin secretion and GnRH self-priming were studied in incubated hemipituitaries from rats on each day of the 4-day estrous cycle. hFF inhibited only GnRH self-priming in pituitaries from rats in diestrus. In the second experiment, immunohistochemical PR expression and action were evaluated in pituitaries from rats in diestrus. PR-positive (PR10A9 antibody) gonadotropes were detected (4–5/field 40x), and antiprogestins added to the incubation media blocked the ligand-independent (GnRH) activation of PR effects on GnRH self-priming. Finally, the third experiment evaluated the effects of hFF on P-induced potentiation of GnRH-stimulated LH secretion. GnSAF bioactivity, as evidenced by inhibition of PR-induced potentiation of GnRH-stimulated LH secretion, was found in diestrous pituitaries incubated with hFF. The results indicate that GnSAF attenuated GnRH-dependent LH secretion in diestrus through the inhibition of PR-dependent GnRH self-priming. |
| Databáze: | OpenAIRE |
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