| Autor: |
Michel Maitre, Guy Vincendon, J.F. Rumigny, D. Weissmann-Nanopoulos, Paul Mandel |
| Rok vydání: |
1982 |
| Předmět: |
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| Zdroj: |
Neurochemistry International. 4:523-529 |
| ISSN: |
0197-0186 |
| DOI: |
10.1016/0197-0186(82)90041-9 |
| Popis: |
Hydroxybutyric acid, a reductive catabolite of GABA, has numerous neuropharmacological and neurophysiological properties when injected systematically to animals. Recently, a specific succinic semialdehyde reductase (SSR2) has been isolated from rat brain. This enzyme specifically produces [(3)H]?-hydroxybutyrate from [(3)H]GABA when incubated in vitro with rat brain tissue slices. A specific antibody against this enzyme has been raised in the rabbit and employed to localize by immunocytochemical procedures the sites of ?-hydroxybutyrate synthesis in two regions of rat brain, the nucleus Raphe dorsalis and the median hypothalamus. Light microscopy reveals the presence of numerous SSR2-positive reactions in the cytoplasm of fusiform or ovoid cells. High magnification shows that only neurons of varous sizes are stained; the cytoplasm is uniformly labelled with a few punctate deposits. At the electron microscopic level, some staining appears in the somata of neurons and in fibres or axonal terminals. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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