The role of GDNF in patterning the excretory system
| Autor: | Vivette D. D'Agati, Reena Shakya, Nikolai Kholodilov, Frank Costantini, Zaiqi Wu, Eek-hoon Jho, Pille Kotka, Robert E. Burke |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
medicine.medical_specialty
Kidney development Hoxb7 Mesenchyme animal diseases Organogenesis Restriction Mapping Mice Transgenic Kidney Mesoderm Mesonephric duct Mice Paracrine signalling Ret receptor tyrosine kinase Internal medicine medicine Glial cell line-derived neurotrophic factor Branching morphogenesis Animals Wolffian duct Glial Cell Line-Derived Neurotrophic Factor Nerve Growth Factors Molecular Biology In Situ Hybridization Body Patterning Homeodomain Proteins Metanephric mesenchyme Ureteric bud biology urogenital system Metanephric kidney Gene Expression Regulation Developmental Cell Biology GDNF Epithelium Cell biology Endocrinology medicine.anatomical_structure nervous system biology.protein Ureter Ret Developmental Biology |
| Zdroj: | Developmental Biology. (1):70-84 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2005.04.008 |
| Popis: | Mesenchymal–epithelial interactions are an important source of information for pattern formation during organogenesis. In the developing excretory system, one of the secreted mesenchymal factors thought to play a critical role in patterning the growth and branching of the epithelial ureteric bud is GDNF. We have tested the requirement for GDNF as a paracrine chemoattractive factor by altering its site of expression during excretory system development. Normally, GDNF is secreted by the metanephric mesenchyme and acts via receptors on the Wolffian duct and ureteric bud epithelium. Misexpression of GDNF in the Wolffian duct and ureteric buds resulted in formation of multiple, ectopic buds, which branched independently of the metanephric mesenchyme. This confirmed the ability of GDNF to induce ureter outgrowth and epithelial branching in vivo. However, in mutant mice lacking endogenous GDNF, kidney development was rescued to a substantial degree by GDNF supplied only by the Wolffian duct and ureteric bud. These results indicate that mesenchymal GDNF is not required as a chemoattractive factor to pattern the growth of the ureteric bud within the developing kidney, and that any positional information provided by the mesenchymal expression of GDNF may provide for renal branching morphogenesis is redundant with other signals. |
| Databáze: | OpenAIRE |
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